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Effect of 2, 3, 5, 4’-tetrahydroxystilbene-2-O-β-D-Glucoside on Apoptosis of Endothelial Cells Induced by H2O2

Author: HuangLiangZhu
Tutor: LongShiYin；TianYing
School: Nanhua University
Course: Biochemistry and Molecular Biology
Keywords: Human vein endothelial cell 2, 3, 5, 4′-Tetrahydroxystilbene-2- O-β-D- glucoside apoptosis PARP Caspase-3 Bcl-2 Bax
CLC: R285.5
Type: Master's thesis
Year: 2011
Downloads: 32
Quote: 0
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Abstract


Aim: To observe the cytoprotection effects and molecular mechanisms of 2,3,5,4′-tetrahydroxystilbene-2-O-β-D-glucoside on the apoptosis of human vein endothelial cell induced by H2O2.Methods: Viability of HUVECs was detected by MTT. The morphorlogy of apoptosis was obversed by Hoechst33258 staining and apoptosis rate of HUVECs was detected by Flow Cytometry. The mRNA expression of Caspase-3, PARP was detected by RT-PCR and the protein expression of Caspase-3, PARP, Bcl-2 and Bax was detected Western-blotting.Results: The cell viablity decreased and the cell apoptosis rates increased with the increasing concentration of H2O2.The apoptosis rates were 6.3±0.03%, 14.9±0.2%, 25.3±0.3%, 28.8±0.6%, 33.0±1.1% respectively. The cell viablity increased after pretreated with TSG （10,100μmol/L） （P<0.05）. The apoptosis rates were 18.33±2.60%, 16.16±1.96%, 15.0±2.35%, 12.43±1.37% in TSG groups respectively, which were all lower than 300μmol/LH2O2 group significantly（except for 0.1μmol/LTSG group, P<0.05）. The apoptosis cells decreased in TSG groups, especially in 10 and 100μmol/LTSG goups. Compared with H2O2 group, the gene expression of Caspase-3 and PARP was decreaced, the protein of Bax was decreased while the protein of Bcl-2 was increased and the ration of Bcl-2 and Bax was promoted in TSG group significantly （P<0.01）.Conclusion: TSG could inhibit the apoptosis of HUVECs induced by H2O2, and the mechanism might be related to decrease the expression of Caspase-3, PARP and Bax, and increase the expression of protein Bcl-2.

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