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Functional ingredients tea Mechanism of in vitro lipid

Author: HuKai
Tutor: HuangHuiHua
School: South China University of Technology
Course: Of Food Science
Keywords: Green tea Polyphenols Tea extract Cholate Combine
CLC: R285
Type: Master's thesis
Year: 2011
Downloads: 264
Quote: 2
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Abstract

In this thesis, tea vitro binding ability of bile salt tea lowering effect as judged by indicators, the main research in vitro lipid-lowering effect of tea and mechanism. Clear bile salts detection method; studied the adsorption of tea on bile salt kinetics and adsorption isotherm; were different compared different tea powder and tea extract in a certain pH, temperature conditions, combined with certain bile salts capacity; through correlation analysis to determine the initial binding bile salts in tea major functional components, and polyphenols, caffeine pure product was tested; analysis of the impact of tea and its functional components related factors combined bile salts, preliminary judgment tea and functional ingredients combined with the mechanism of bile salts. By UV spectrophotometry three kinds of bile salts, the standard curve R ~ 2 sodium taurocholate were 0.9989, 0.9977 sodium glycocholate, cholate 0.9993, detection methods are stability and reproducibility better. Through the combination of bile salts tea kinetic curves and adsorption isotherm analysis shows that the combination of green tea on bile salts faster, at the beginning of the adsorption equilibrium has been reached within 30min amount of 90%, and the green and bile salts The binding reaction free energy of adsorption △ G are negative, which means that the response of green tea with bile salts is spontaneous reaction; Freundilich isotherm, Langmiur isotherm, Tempkin isotherm three kinds isotherm can be used to describe green tea bile acid binding behavior, the correlation coefficient r gt; 0.97; green tea on sodium taurocholate, sodium glycocholate and other conjugated bile salts than the combined effects of bile salts on the free type combined effects of sodium cholate more Well, may be due to the conjugated bile salts with polar side chains, more conducive to active sites of combined exposure to bile salts, theoretical analysis and experimental results. In vitro human digestive environment to the main component of lipid-lowering drugs cholestyramine as controls, and comparison of different tea extract bile salts in vitro binding capacity. The results show that the ability of green tea combined with bile salts strongest green tea powder combined with sodium taurocholate, sodium glycocholate, sodium cholate amount equivalent to the same dose of cholestyramine for 40.2%, 45.7%, and 69.7 %; green tea extracts combined with sodium taurocholate, sodium glycocholate, sodium cholate amount is cholestyramine 38.4%, 46.5% and 42.0%. Followed by a semi-fermented tea (Iron Goddess of Mercy) and fermented tea (tea, Dianhong tea and six Fort tea). Where green tea on the binding capacity of bile salts was significantly stronger than ordinary fruits and vegetables, indicating that green tea has a prominent hypolipidemic effect. Proved by experiments with tea polyphenols binding ability of the bile salt, two showed a significant positive correlation with polyphenol content in different tea powder on sodium taurocholate, sodium glycocholate, sodium cholate combined amount of correlation coefficients were 0.851,0.953,0.904 different tea polyphenols extract bile salt content of its combination of three kinds of correlations were 0.939,0.841,0.895, higher content of tea polyphenols , the combination of the stronger bile salts. Caffeine and green tea extract combined with the impact of certain bile salts, bile salts help to integrate, but the impact was significantly less effective than polyphenols, which combines pure bile salts was significantly lower than the amount of polyphenols pure product ( P ≤ 0.05), crude fiber content in tea combined with the amount of bile salts were negatively correlated, but the correlation between the two is not significant. pH on green tea extracts and tea polyphenols binding bile salts were significantly affect the ability of urea added green tea extract combination affects the amount of bile salts, changing the ionic strength of the buffer solution on the combination of green tea extract The amount of bile salt was not significant (P ≥ 0.05), show that electrostatic interactions functional ingredients in green tea, the combination with the bile salt plays an important role in the process of hydrogen bonding in the bile salt and green tea extracts and tea The combination of polyphenols play a role in the process, hydrophobic interaction functional ingredients in green tea, combined with bile salts in the process no significant contributions.

Functional ingredients tea Mechanism of in vitro lipid

Abstract

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