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Study on the Extraction of Essential Oil from Rhodomyrtus Tomentosa (Ait.)hassk and the Effect on the Activity of Nitrite Reduction

Author: ChenWenFen
Tutor: ZhaoMouMing
School: South China University of Technology
Course: Of Food Science
Keywords: Rhodomyrtus tomentosa (Ait.) Hassk essential oil SFE activity of nitrite reduction
CLC: R284
Type: Master's thesis
Year: 2011
Downloads: 95
Quote: 0
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Abstract


The research used Rhodomyrtus tomentosa （Ait.） Hassk as raw material, which was distilled by SFE（CO2） for essential oil,and the extraction condition was optimized by RSM. The antioxidant activity and the activity of nitrite reduction of essential oil in the ethanol-water solution were studied. The relationship between the antioxidant activity and the activity of nitrite reduction of essential oil was searched, which was validated by oxidation-reduction poten-tial. The essential oil was used to Cantonese sausage to reduce the content of nitrite.According to the extraction efficiency and the activity of nitrite reduction, four different kinds of extraction methods （ Hydro-distillation, Enzyme-distillation, Organic solvent-distillation, SFE） were studied. The results were as following respectively: Hydro-distillation , the extraction efficiency was 0.37%,and the activity of nitrite reduction was 29.21%; Enzyme-distillation, 0.42% and 13.42%;Organic solvent-distillation,1.02% and 10.60%; SFE,6.31% and 19.70%. Their optimization conditions were obtained as: pressure, 20MPa; temperature, 50℃; time, 1.5h;co-solvent, 0.75mL/g. The the extraction efficiency and the activity of nitrite reduction were 5.68% and 19.50% in this condition.The relationship between the antioxidant activity and the activity of nitrite reduction of essential oil in the ethanol-water solution was searched, which was compared with Vc. The content of nitrite in the samples were determined every 24h. The results showed that:the essential oil could be dissolute in the ethanol-water solution with ethanol concentration >50%. The antioxidant activity of essential oil was evaluated by in vitro experiments using scavenging assays of DPPH·radicals, ABTS·+ radicals, superoxidant anion radicals,and reducing power,respectively.The essential oil showed strong radical scavenging activity.The processing of the activity of nitrite reduction was studied in detail,which was traced by oxidation-reduction poten-tial.It was confered that the processing of the activity of nitrite reduction was homeostasis. The activity of nitrite reduction was 19.82%.The essential oil was used to Cantonese sausage to reduce the content of nitrite, and the essential oil showed favorable application effect.

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