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Effect of Curcumin on Expression of Cathepsin B and Cystatin C in Rats with Alcoholic Liver Diseases

Author: ChenYao
Tutor: WanYuanTai
School: Wuhan University of Science and Technology
Course: Epidemiology and Biostatistics,
Keywords: hepatocytes apoptosis curcumin Cat B gene Cys C gene
CLC: R285.5
Type: Master's thesis
Year: 2011
Downloads: 30
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Abstract


Objective:To investigate the effect of curcumin on expression of Cat B and Cys C in rat with alcoholic liver diseases.Methods:1. 42 male SD rats were randomLy divided into three groups:12 rats for normal group. Both 15 rats for model and CUR groups. Alcoholic liver diseases were induced by gavage.When 6 W, CUR group with alcohol plus CUR.2. Rats were killed at the end of 12W. Liver coefficient of the rats were calculated. Levels of serum aspartate aminotransferase(AST), alanine aminotransferase(ALT) were measured. To calculate those AST/ALT.3. The pathological changes of liver was observed after H-E.4. The protein expression of Cat B/Cys C were detected by immunohistochemistry and Western Blot.5. The mRNA expression of Cat B/Cys C were determined by RT-PCR.Result:1. Compared with that in the normal group, liver coefficient increased significantly in the model group (P<0.01). To compare with the model group, the liver coefficient of CUR group decreased (P<0.01).2. LM observed that the hepatocytes became swelling and macro or microvesicular steatosis, inflammatory cells infiltration were observed in the portal tract, and microvesicular fatty change obviously in the model group. But the hepatocytes were injured slightly in the CUR group.3. The levels of serum AST and ALT increased significantly in the model group (P<0.05) compared with that in the normal group. To compare with the model group, the levels of AST and ALT decreased significantly in the CUR group(P<0.05), and that increase slightly compared with the normal group(P>0.05) .4. The protein expression of Cat B and Cys C in the liver detected by immunohistochemistry and western blot increased significantly in the model group(P<0.01) compared with that in the normal group. And to compared with the model group, Cat B and Cys C expression decreased significantly in CUR group(P<0.01). 5. Cat B and Cys C expression in the liver determined by RT-PCR increased significantly in the model group(P<0.01) and increased slightly in CUR group(P>0.05) compared with that in the normal group . But to compared with the model group, Cat B and Cys C expression decreased significantly in CUR group(P<0.01).Conclusion:CUR can diminish alcohol-induced liver injury in rat through decreasing the expression of Cat B and Cys C and inhibiting the hepatocytes apoptosis by alcohol.

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