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Effects of Ginsenoside Rg3 on Proliferation of Cultured Human Lens Epithelial Cells

Author: ZhaoYing
Tutor: LiuDan
School: Liaoning Medical
Course: Ophthalmology
Keywords: Human lens epithelial cells SRA01/04 Ginsenoside Rg3 Apoptosis
CLC: R285.5
Type: Master's thesis
Year: 2011
Downloads: 46
Quote: 0
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Abstract


Objective To investigate the ginsenoside Rg3 (ginsenoside Rg3) on cultured human lens epithelial cells (Human lens epithelial cells, HLECs) SRA01/04 proliferation and apoptosis of them . Methods Cultured human lens epithelial cells SRA01/04, trypsin digestion 1:2 passage , take the first three cells tested. Different concentrations Rg310, 20,40,80,120,160 μg / mL SRA01/04 cells cultured in vitro 12,24,48 and 72h by MTT colorimetric analysis of its inhibitory effect on cell growth ; AO / EB staining cell morphological changes were observed ; flow cytometry apoptosis rate ; Western blot determination of its expression of Caspase-8 . Rg3 results within a certain range SRA01/04 cell growth inhibition , with the extension of time when the drug concentration, its inhibition enhanced SRA01/04 cells in a time dependent concentration . MTT visible at the same time , the inhibition rate of the different experimental groups was significantly (P lt; 0.05), with the mass concentration increases. The same experimental group , the inhibition rates at different times there was significant difference (P lt; 0.05), increased with time ; AO / EB staining nuclei with the experimental group SRA01/04 fluorescent yellow or orange-red fluorescence, nuclei were solid shrink -like or bead-like , apoptosis was significantly changed ; flow cytometry apoptosis rate increased with the concentration of drugs ; Western blot shows Caspase-8 cells in treatment group compared with control group with high expression . Conclusion Rg3 significantly inhibited cell proliferation SRA01/04 and time- and concentration -dependent ; certain concentration SRA01/04 Rg3 can induce apoptosis and cell apoptosis rate increased with the increase of drug concentration ; Rg3 by upregulating the expression of Caspase-8 protein induces cell apoptosis ; prompt Rg3 may become effective after cataract prevention drugs.

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