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Purpose:The experiment by establishing chronic heart falling (CHF) model rats, and observe chinese medicine the benefiting Qi and activating Blood compound to CHF rats expression of ColⅠ, ColⅢ. From protein learn, genes, and organizational forms to learn and study its function and role of the mechanism; for the chinese medicine prevent and treat CHF to open up new ideas and objective scientific experimental basis.Material and method:1. Male common grade 60 SD rats of weighing from 200～230g were used,and applicably feed on a week. CHF model rats model by ligating coronary artery with exhaustive style swimming , hunger and other methods, then detecte ejection fraction (EF) and so on by Doppler echocardiography, and the status of the rats, determine the success of the modeling.2. To successful CHF model rats divided into western medicine group,traditional chinese medicine group, model group, and set up the blank control group. Blank control group and model group did not medicate, only fed saline water of conventional volume, rats in medication groups taked medicine by ratand men dose formula , western medicine group dosed La nuo pu li; traditional chinese medicine group dosed Yi Qi Huo xue Compound prescription.6 weekslater , all groups rats were killed after anesthesia, left the heart myocardium.3. Using real time quantitative PCR, Western blot method,and HE staining and immunohischemistry observed under light microscope to compare the chinesemedicine group,western group and blank control group, model group with CHF cardiac ColⅠ, ColⅢand the changes in morphological changes among groups. Results:1. Chronic heart failure animal model group ColⅠ, ColⅢmRNA expression was significantly decreased ,compared with the control group P<0.01; except western group ColⅠmRNA slightil decreased,compared with the model group P>0.05;by drugs intervention ColⅠ, ColⅢmRNA of each treatment group was significantly increased, compared with model group P<0.01;compared with Chinese medicine group statistically significant,P<0.01.2. CHF model group, the expression of Col,ColⅢwas significantly decreased,compared with the control group P<0.01; by drugs intervention ColⅠ, ColⅢexpression of each treatment group was significantly higher, compared withthe modle group P<0.01; ColⅠchinese medicine group compared with western medicine group medicine group,P<0.01;ColⅢchinese medicine group compared with western medicine group medicine group,P>0.05.3. The myocardium cells of blank control group:regular arrangement, normal size ,stripes clear, and orderly arrangement of myofibrils. Model group:myocardium cells formed extensive networks, can not observe clear stripes and disordered myofibrils, the blank area heterochromatin present mass, adjacent cells in the intercalated disk space widened.Western medicine group :myocardium cells arranged in regular , most of the myocardium cells on the edgeof myocardium cells, shape, size normal and more orderly arrangement ofmyofibrils.Chinese medicine group: myocardium cells arranged in regular, can observe clear stripes, myocardium cells were shrunk to a more solid round,onthe edge of myocardium cells, a more orderly arrangement of myofibrils stillshowde that there were faults and defects and the blank area.4. The average gray of CHF model group ColⅠanalyzing by software was 45.34±8.10 ,using immunohistochemistry ,compared with the control group P<0.01;Chinese medicine group impression significantly was 468.86±9.87,compared with model group, P <0.01; Western group was 396.20±39.95 ,compared with model group, P<0.01,there is significant difference among western group and chinese medicine,P>0.05.5. The average gray of Chronic heart failure model group ColⅢanalyzing b y software was 61.46±7.11, using immunohistochemistry,compared with the blank control group P<0.01; Western group was the most obvious , 132.40±55.34 ,compared with the model group, P<0.01; Chinese medicine group was 108.26±8.89,compared with the model group, P<0.01;there is significant difference among western group and chinese medicine P>0.05.Conclusion:1. Through the rats use a combination of the coronary artery ligated, hungry, and exhausted swimming,methods, ultrasound echocardiography detected by the rats and left ventricular function of its actions, prove that the changes made by the method is feasible.2. After 6 weeks treatment, by using Western blot, real time quantitative PCR, HE method dyeing detect ColⅠand ColⅢcontent of CHF rats myocardial tissue , found that ColⅠand ColⅢContent of Yi qi huo xue compound prescription was significantly higher than the model group myocardium tissue,and precede western medicine group ;furthermore,it can improve myocardium structure;to clue:Yi qi huo xue compound prescription can regulate myocardium ColⅠand ColⅢcontent and improve myocardium structure , thus to prevent and treat function of CHF.
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