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Objective: To observe rehabilitation training combined with electro-acupuncture treatment of stroke limb spasticity Synaptic binding protein I expression to explore rehabilitation training combined with electro-acupuncture treatment of post-stroke hemiplegic limb spasms mechanism. Methods: 75 SD rats were randomly divided into five groups: control group, model group, rehabilitation training group (rehabilitation group), electro-acupuncture group (EA group) and treatment group (the consolidated group). By suture copy of focal cerebral ischemia in animal models. After the modeling third day, respectively rehabilitation training, electro-acupuncture treatment, rehabilitation training combined with three different electro-acupuncture treatment, treatment times a day, six days of continuous treatment. Rats in each group after 1 day, 3 days, 9 days Bederson neurological score to assess nerve function, balance beam walking score, the Screen test evaluation to assess motor function, muscle tension changes observed before and after treatment. After the end of treatment, rats were sacrificed by immunohistochemical method for the determination of the rats brain stem, spinal cervical enlargement and lumbar enlargement of synaptic expression of the protein I (SYT I). Results: 1. Bederson (?) Neurological function, balance beam walking score, Screen Test evaluation: 1 day after surgery, compared with the control group, model group and treatment group rating scores were significantly higher, and the difference was statistically significant ( P lt; 0.05), but no significant difference between the group (P gt; 0.05) After 3 days, model group and treatment group rating scores were lower trend, but the difference was not statistically significant (P gt; 0.05) Nine days after surgery, compared with model group, the rehabilitation group, EA group, consolidated group rating scores were significantly lower (P lt; 0.05), an integrated set of the most obvious (P lt; 0.05) (2) assessment of muscle tone: before treatment, compared with the control group, model group, the treatment group muscle tension increased in varying degrees, the difference was statistically significant (P lt; 0.05), but no significant difference between the groups (P GT ; 0.05). After treatment, compared with model group, the rehabilitation group, EA group, consolidated group muscle tone has improved, the differences were statistically significant (P lt; 0.05), the most obvious effect of a consolidated group improved (P lt; 0.05 ). Expression of 3.SytI: Compared with the control group, the model group, the rat brainstem, spinal cervical enlargement, lumbar enlargement Syt I expression was significantly reduced (P lt; 0.05); compared with the model group, the rehabilitation group, electro-acupuncture group, comprehensive set of rat brain stem, spinal cervical enlargement, lumbar enlargement in SYT I expression was significantly increased (P lt; 0.05), the most obvious to the increase of the consolidated group (P lt; 0.05). Conclusion: 1. Rehabilitation training, electro-acupuncture treatment, rehabilitation training combined with electro-acupuncture treatment can improve the neurological and motor functions of stroke limb spasticity, rehabilitation training combined with electro-acupuncture treatment better. Rehabilitation training, electro-acupuncture treatment, rehabilitation training combined with electro-acupuncture treatment can reduce stroke limb spasticity rat muscle tone, and better rehabilitation combined with electro-acupuncture treatment. Rehabilitation training, electro-acupuncture treatment, rehabilitation training combined with electro-acupuncture treatment can increase the expression of syt I, rehabilitation training combined with upregulation of electro-acupuncture treatment effect is more obvious. Rehabilitation training combined with electro-acupuncture treatment can promote the expression of Syt I, which may be one of the internal mechanism of rehabilitation training combined with electro-acupuncture treatment to ease the stroke limb spasticity.
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