Dissertation > Excellent graduate degree dissertation topics show

Inheritance of Stripe Rust Resistance Gene and Identification of HMW-GS in Thinopyrum-derived Wheat Breeding Lines

Author: BaiYun
Tutor: ChangZhiJian
School: Shanxi University
Course: Crop Genetics and Breeding
Keywords: Wheat Thinopyrum ponticum Stripe rust resistance HMW-GS Molecular markers
CLC: S512.1
Type: Master's thesis
Year: 2011
Downloads: 1
Quote: 0
Read: Download Dissertation

Abstract


The ongoing improvement of wheat cultivars is dependent on a continuous supply of genetic variability. The Thinopyrum species, Th. intermedium (2n=6x=42) and Th. ponticum (2n=10x=70) both carry many useful agronomic traits. Partial wheat-Thinopyrum amphiploids are potential tertiary gene pools for wheat improvement. An attempt was made to transfer resistances to powdery mildew and yellow rust into wheat from Th. intermedium and Th.ponticum, and many multi-resistance lines including CH7103 have been developed by crossing susceptible wheat cultivars with resistant partial amphiploids as donor parents. The objectives of this study were to determine the inheritance of stripe rust resistance gene introgressed from Th. ponticum in wheat line CH7103 and its allelism with known resistance genes, Yr5, Yr10, Yrl5 and Yr41, for resistance to a prevalent and most widely virulent race CYR32 in China, and to analyze the compositions of high molecular weight glutenin subunits (HMW-GS) in ninety-three Th. intermedium-derived lines. The results are as follows:1. CH7103 is a wheat multi-resistance line developed recently from crossing susceptible wheat cultivars with Thinopyrum ponticum-derived resistant partial amphiploid Xiaoyan7430 as donor parent. In this study, the evaluation of resistance reactions demonstrated that CH7103 and its donor Xiaoyan7430 as well as the wild parent were resistant at the seedling and adult stages to CYR31 and CYR32 of the prevalent and most widely virulent in China, whereas all the wheat parents involved were susceptible, indicating that the stripe rust resistance in CH7103 was possibly derived from Th. ponticum. Genetic analysis of the F1,F2, F2:3 and BC1 populations from stripe rust resistant line CH7103 revealed that a single dominant gene was responsible for the adult resistance to CYR32. The allelism tests of four F2 populations demonstrated that the stripe rust resistance gene in CH7103 was likely different from the known resistance gene Yr5, Yr10, Yrl5 or Yr41. The cytological observation showed that the somatic chromosome number of CH7103 and its F1 hybrids involving T. aestivum cv. Chinese Spring and Taichang29 was 2n=42, and their most pollen mother cell had a regular pairing of 2n=21Ⅱat meiosis, suggesting that this resistant line was a stable cytological cryptic alien introgression with being largely identical to the chromosome constitution of breed wheat.2. Using SDS-PAGE technology to analyze the compositions of HMW-GS in 93 wheat lines from the cross of common wheat with Th. intermedium-derived partial amphiploid TAI7045, then to use PCR-based markers for confirmation of Aχ2*, Dχ5, Dy10, Bχ14, Bχ17, at Glu-Al, Glu-Bl and Glu-Dl loci, respectively. The SDS-PAGE results showed the proportion of high quality subunits was relatively smaller and ten kinds of allelic variation at site Glu-1 in lines tested. Among them, the N type was main in N,1,2* encoded at Glu-Al; the frequency of 7,17+18 type was high at Glu-Bl loci; and 2+12 was the main type of glutenin subunits at Glu-Dl. A total of eleven kinds of HMW-GS combination types were detected and the frequency of subunit compositions N,7,2+12 and N,17+18,2+12 was higher. Three lines had excellent quality subunits at 2 encoding loci, account for 3.3%, and five lines had excellent quality subunits at 3 loci, account for 5.4%. The quality scores of eight lines were 8-10, account for 5.4% of all the tested genotypes. The results of PCR-based markers identified the frequency of Ax2*, Dx5, Dy10, Bxl4 and Bxl7, which were consistent with those of SDS-PAGE analysis.

Related Dissertations

  1. Functional Analysis of Proteins Encoded by RNA2 of Wheat Yellow Mosaic Virus,S435.121
  2. Pathogenic Differentiation and Genetic Diversity of Wheat Sharp Eyespot in Henan Province,S435.121
  3. The Identificationand Rapdanalysis of Wheat Root Lesion Pathogens in Henan Province,S435.121
  4. Nitrogen Regulation and Management on High-yield Wheat-maize Rotation System,S512.1
  5. Effect of Nitrogenapplication Patterns on Variation of Soil Nitrate-N and Grain Yield of Wheat and Maize,S512.11
  6. Study on Nitrogen Accumulation Characteristics of Winter Wheat with Yield of 10t/ha, Diagnosis and Nitrogen Application Technologies,S512.1
  7. Studies on the Life Cycle and Classification of Agrocybe Salicacola,S646
  8. Optimization of Fermentation Conditions of Recombinant Lipoxygenase for the Production in Wheat Flour,TS201.25
  9. Study on Extraction,purification and Antioxidant Activity of Wheat Germ Polysaccharides,TS210.1
  10. Characterization of Mineral Phosphate-Solubilizing Bacterium K3 and Colonization under Different Soil,S144.9
  11. Design and Implementation of the Growing Visualization System for Wheat Population,S512.1
  12. Trajectory Analysis of Wheat Aphids During Long-distance Migration Using HYSPLIT Model,S433
  13. Genetic Structure of the Wheat Powdery Mildew Populations from Henan, Hubei and Chongqing Province (City),S435.121
  14. Cloning 1Dx5 Gene from the Xinjiang Wheat Variety and Constructing Its Expression Vector,S512.1
  15. Studies on Regulating Effect of Nitrogen Rates on Starch Granule Distribution in Grain of Wheat,S512.1
  16. Accumulation of High Molecular Weight Glutenin Subunits in Wheat Grain as Regulated by Nitrogen Rate,S512.1
  17. Effects of Pre-Anthesis Drought Hardening on Grain Yield and Quality Formation in Wheat under Post-Anthesis Drought and Its Physiological Mechanism,S512.1
  18. Difference in Development between Culm and Tiller Roots and Their Contribution to Grain Yield and Quality in Wheat (Triticum Aestivum L.),S512.1
  19. Laws of Physiological Characteristics,dry Matter Accumulation and Transfer of Population After Anthesis in Two Kinds of Different Wheat Varieties,S512.1
  20. Differences in Winter Wheat Productivity and Nitrogen Use Efficiency between the Main Varieties Released in Different Times in Jianghuai Area,S512.1
  21. Quantifying the Responses of Weak Gluten Wheat Leaf Photosynthesis and Chlorophyll Fluorescence Parameters to Weak Light at Filling Stage,S512.1

CLC: > Agricultural Sciences > Crop > Cereal crops > Wheat > Wheat
© 2012 www.DissertationTopic.Net  Mobile