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Objective animal experiments observed grass particles in water treatment of recurrent oral ulcers (ROU), ELISA determination of interleukin-4 (IL-4) concentrations, and inflammatory changes in the status of research treatment of Shuizhongcao particles ROU process, ELISA measurement of interleukin 4 (IL-8), granulocyte - macrophage colony stimulating factor (GM-CSF) concentration of neutrophils decreased immunological mechanisms clarify treatment of Shuizhongcao particles ROU process reversed. ① 70 healthy clean SD rats, male and female, weight 180 ± 20g, 6 ~ 8 weeks of age, were randomly divided into normal control group, 0.9% NS treatment control group, Shuizhongcao five days of treatment group, Shuizhongcao therapy 10 day group treated for 15 days group and water grass. In addition to the normal group, the rest of the group, the three days fluorouracil (5-FU) 50mg/100g the dose intraperitoneal chemotherapy each time, the fourth day of the 10% chloral hydrate anesthetized by intraperitoneal injection 0.3ml/100g dose SD rats hemostat separate upper and lower jaws, fixed flat toothed forceps, gently pull out the right side of the oral mucosa, the tweezers NaOH crystals, of a diameter of approximately 5mmm accurately placed on the right side of the buccal mucosa at fixed continuous contact for 5-10 seconds, in order to be seen with the naked eye the mucosal ulceration bleeding prevail, oral ulcers model. ② of normal oral mucosa injury, do the biopsy, observation of oral ulcers damaged and the recovery of ③ The normal diet are to be observed each group of rats with general signs and symptoms and mouth ulcers changes, 6, 11, 16 days of treatment rats sacrificed corresponding serum 1L-4, IL-8 and GM-CSF content. Results ① oral mucosal histopathologic observation: the normal group oral mucosa finishing no obvious inflammatory cell infiltration. Remaining rats ulcers oral mucosa visible mucosal epithelial ulceration, shedding the formation of ulcers, the surface fibers exudate and necrotic cells, inflammatory cells together to form a pseudomembrane coverage, inflammatory cells mainly neutrophils and lymphocytes, followed by plasma cells. The treatment group ② Shuizhongcao compared with the saline control group, generally better recovery, mouth ulcers to heal fast 1-2 days. ③ by ELISA, in the serum IL-4, IL-8 and GM-CSF content Shuizhongcao treatment of IL-4 content of the 10-day group increased significantly compared with the other groups, P lt; 0.05; Shuizhongcao treatment 10 days, 15 days of IL-8 group compared to the other group content decreased significantly, P lt; 0.05; Shuizhongcao content increased significantly compared to the other groups of GM-CSF treatment group five days, P lt; 0.05. Conclusion 1. Shuizhongcao particles can promote healing mouth ulcers, to improve rats generally. 2 particles of Shuizhongcao to promote the body to secrete IL-4, reduce the secretion of IL-8, inhibit inflammation reaction. The early oral ulcers increased secretion of GM-CSF after chemotherapy, the late GM-CSF decreased, not yet Description Shuizhongcao particles promote GM-CSF. After chemotherapy oral ulcer healing and control of inflammation has an important relationship. Grass particles in water by promoting the elimination of inflammation and speed up the healing of mouth ulcers.
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