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Preliminary Research of the Effects of Sphingosine on Medicago Sativa L. and Cucumber Callus Induction and Resistance

Author: ZhengXiaoJie
Tutor: JiangJiZhi
School: Hebei University
Course: Botany
Keywords: Sphingosine Alfalfa Cucumber Callus Water Stress Low temperature stress
CLC: Q943.1
Type: Master's thesis
Year: 2006
Downloads: 29
Quote: 0
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Abstract


The trial the alfalfa ( golden varieties) and cucumber ( Shen Nong No. 5 and Tianjin sterile No. 1 variety) were used to study the sphingosine (sphingosine) Treatment of alfalfa and cucumber callus induction and differentiation , as well as in drought and temperature stress on some physiological indicators . The results show that early in the induction of both is less than 0.1mg / L sphingosine alfalfa processing, or processing of 0.15mg / L, 0.3mg / L and 0.6mg / L concentration sheath ammonia alcohol cucumber callus callus induction had no significant effect . Cucumber varieties , 0.6mg / L the sphingosine processing can promote cucumber callus redifferentiation . Sphingosine callus SOD, POD activity . 0.06mg / L sphingosine treatment of alfalfa callus SOD activity was significantly higher , 0.02mg / L sphingosine processing POD activity can be significantly increased . Sphingosine cucumber callus , early callus induction of SOD and POD activity reached its peak , but the late induction tends to decrease . Osmotic stress of polyethylene glycol (PEG-6000) analog , alfalfa callus POD activity , cucumber callus SOD activity and free proline content were higher in the treatment group of sphingosine . Water Stress 72h, the sphingosine processing makes the alfalfa callus soluble sugar and protein content increased processing of 0.06mg / L concentration of sugar and protein content increased by 10% and 25% , respectively . Cucumber callus malondialdehyde (MDA) in the treatment of the early stage of water stress by sphingosine changes slowly , after the big variations ; chilling stress, an increase compared with the control group, MDA content 0.6mg / L sphingosine treatment fast , sphingosine can be reduced under low temperature stress of MDA accumulation , and enhance the adaptability of plants to low temperature . Water stress , cucumber callus protein kinase activity was increased and then decreased enzyme activity peak in time treatment group compared with the control night, the inhibition of protein kinase sphingosine certain extent . These can be in the treatment of a certain concentration of sphingosine callus of alfalfa and cucumber plants increase their adaptability in the face of adversity which improve stress resistance .

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CLC: > Biological Sciences > Botany > Plant Cell Genetics > Plant cells in vitro culture
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