Dissertation > Excellent graduate degree dissertation topics show

Coenzyme NAD (P) H in the autofluorescence of cells observed

Author: ChenRong
Tutor: ZhouLuWei；ChenZuoYao
School: Fudan University
Course: Condensed Matter Physics
Keywords: NAD(P)H autofluorescence oscillation patterns RBL-2H3 cells sensitive temperature
CLC: Q55
Type: Master's thesis
Year: 2008
Downloads: 74
Quote: 0
Read: Download Dissertation

Abstract


Nicotinamide adenine dinucleotide phosphate (NAD(P)H) is an important coenzyme involved in the energy metabolism of living cells. NAD(P)H binds to proteins associated with both aerobic and anaerobic metabolism.The spatial and temporal distributions of cellular NAD(P)H concentrations are theoretically predicted as typical patterns of the metabolism in living cells, and so far only be observed in neutrophils. By fast imaging the autofluorescence of the cellular NAD(P)H with a sensitive CCD detector in a confocal microscope, the cytoplasmic NAD(P)H wave traveling along the long axis of the elongated cell was measured in Rat basophilic leukemia (RBL-2H3) cells. While for human hepatocellular carcinoma (HepG2) cells, the NAD(P)H mainly localized in mitochondria. The oscillation of the mitochondrial NAD(P)H may be the signs of the metabolic pattern in these HepG2 cells.In addition to neutrophils, we reported here that the cytoplasmic NAD(P)H wave also can be observed in RBL-2H3 cells, supporting the prediction that the NAD(P)H wave is typical metabolic pattern in living cells. However such NAD(P)H wave is NAD(P)H distribution dependent. When NAD(P)H distribute in cytoplasm of the cell, the cytoplasmic NAD(P)H wave could be formed. While for the cells that the NAD(P)H mainly localize in the mitochondria, the oscillation of mitochondrial NAD(P)H may become the metabolic pattern instead of the cytoplasmic wave. The concentration of cellular NAD(P)H is generally weak. Employing more sensitive CCD detector, the NAD(P)H involved metabolic patterns may be observed in other cell lines to gain the knowledge for understanding the metabolism and visually reveal the metabolic regulations in different living system.Besides, the autofluorescence of NAD(P)H in RBL-2H3 cells is sensitive to the environment temperature. Generally, the intensity of cellular NAD(P)H autofluorescence is weaker with the temperature decreasing, which is similar to the intensity change for pure NAD(P)H solution. Cells in different life period have not the same results to adjust the environment changes. The intensity gap between different temperatures for growing cells is much large that for normal cells, whose reason may be status of metabolism.

Related Dissertations

  1. Studies on Hypersensibility of Traditional Chinese Medicine Injections Such as Shuanghuanglian with RBL-2H3 Cells,R285
  2. Synthesis, Characterization and Application in Drug Release of pH-and Tempareture-sensitive Hydrogel Beads/microparticles,R94
  3. Investigation on the Preparation and Application of pH/Temperature-Sensitive Sodium Alginate/Poly(N-Isopropylacryla-Mide) Hydrogels Microcapsulation,R944.5
  4. The Synthesis, Structures, Properties and Phase Transitions of Thermo-pH Sensitive Copolymer-liposome,O631.3
  5. Study on the Synthesis and Properties of Acrylic Acid Series Sensitive Hydrogel,R318.08
  6. The ionic strength of the microwave plasma initiated polymerization P (AMPS / NIPA) binary gel performance impact,TQ316.31
  7. Preparation and Characterization of pH and Temperature Sensitive Nanogels,R94
  8. The Rectification Tower of neural network control method,TP183
  9. Effect of Mutation at the Lid Subdomain of Penicillium Expansum Lipase on Its Activity,Q55
  10. Improving Thermostability of the Penicillium Expansum Lipase by Molecular Mutagensis,Q55
  11. The Influence of Dynamic High Pressure Microfluidization on Activation and Conformational Changes of Lipase,Q55
  12. Studies on the Enantioselectivity and Conformation Changes of Lipases Aided by Molecular Modeling,Q55
  13. Enzymatic Properties of D2 Protease and Establishment ELISA for Detecting D2,Q55
  14. Purification, Biochemical and Toxicological Characterization of Acetylcholinesterase in Delia Antiqua,Q55
  15. Effects of N-Glycosylation on Aspergillus Niger Phytase Characterization,Q55
  16. Study of the Electrochemical Behaviors of Biological Enzyme and Its Catalytic Application,Q55
  17. The Effect of Mitochondrial NAD Kinase on Biological Oxidation Function in Saccharomyces Cerevisiae,Q55
  18. Malachite Green-Phosphomolybdic Heteropoly Acid Determination of Phosphodiesterase Activity and the Impact of Inhibitors,Q55-3
  19. Assay of Alanine α-ketoglutarate Transaminase Activity by Kinetic Analysis of Its Reaction Coupled to Reaction of Lactic Acid Dehydrogenase,Q55
  20. Study on 5’-nucleotidase from Trimeresurus Albolabris Venom of Its Purification、Physicochemical Property and Mechanism of Inhibiting Rabbit Platelet Aggregation,Q55
  21. Novel Approaches to Localize and Quantify Glucose-6-Phosphate Dehydrogenase during Fertilization of Sea Urchin (Psammechinus Miliaris L.),Q55
  22. Structure and Function Studies of a Novel Snake Venom Thrombin-like Enzyme from Agkistrodon Acutus,Q55

CLC: > Biological Sciences > Biochemistry > Enzymes
© 2012 www.DissertationTopic.Net  Mobile