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Study on the Physiological、Biochemical and Molecular Level of Drosophila Melanogaster Treated by High Hydrostatic Pressure

Author: ZhangJie
Tutor: ShenSiLe
School: Jilin University
Course: Biochemistry and Molecular Biology
Keywords: High hydrostatic pressure Drosophila melanogaster Mutation Life RAPD-PCR
CLC: Q967
Type: Master's thesis
Year: 2009
Downloads: 116
Quote: 0
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Abstract


High hydrostatic pressure (high hydrostatic pressure, HHP) is an extreme condition , refers to all higher than the atmospheric pressure conditions . High pressure biology is an interdisciplinary combination of high pressure physics and biological sciences , this high - pressure biotechnology mutagenesis method to study the role of the high pressure of living organisms . Drosophila melanogaster (Drosophila melanogaster) is one of the most thorough human studies biological , and made ??a great contribution to the development of genetics and molecular developmental biology . The following characteristics of Drosophila melanogaster , is particularly suitable as a model organism to study : a relatively short life , genetic and environmental aspects of the operation to change your life , more mature molecular genetic technology , easy to train and analytical testing , has been the complete genome sequence of Drosophila . In this paper, Drosophila melanogaster as the research object , using the the gradient pressure treatment Drosophila eggs . Drosophila life cycle of observation , understanding of high pressure will impact on the life cycle of fruit flies , but also at the molecular level up about this mutagenic . Through experiments that Drosophila physiological and genetic changes caused by high - pressure processing method . At the molecular level using RAPD (random amplified polymorphic DNA) scanning the individual variation of genomic DNA , to obtain a difference in strip proved Drosophila morphology caused by the high pressure treatment , changes in the physiological and biochemical aspects of the change is caused due to the genetic level . This variation is heritable .

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