Dissertation > Excellent graduate degree dissertation topics show

The Research of Molecular Genetics about Carassius Auratus in Qihe River by ISSR and Cytb

Author: GaoLiXia
Tutor: LiXueJun
School: Henan Normal
Course: Ecology
Keywords: C.auratus in Qihe River ISSR Cytb genetic diversity genetic distance
CLC: S917.4
Type: Master's thesis
Year: 2011
Downloads: 4
Quote: 0
Read: Download Dissertation

Abstract


Carassius auratus in Qihe River belonged to Cypriniformes,Cyprinidae ,Carassius Jarocki, which was the natural gynogenesis triploid crucian and produced in Henan province. It is very famous for its rapid growth, excellent taste and high benefits with rosy prospect. Just as these unique features of C.auratus in Qihe River, lots of scholars were interested in the research of its molecular genetics, evolution genetics and so on. This research adopts ISSR and Cytb techniques to analyse genetic diversity and genetic distance among C.auratus in Qihe River and the other C.auratus in wild which in order to konw about genetic evolution and the relations among others near species of C.auratus in Qihe River.According to this research we can offer the theoretical basal for the germplasm resources and genetic breeding.1. ISSR analysis of genetic diversity among C.auratus in Qihe River and the other two C.auratus populations in wildThe study about the genetic diversity of 3 populations which were C.auratus in Qihe River, C.auratus in Jindihe River and C.auratus in Qinhe River by using Inter-Simple Sequence Repeat(ISSR). The results were as follows: (1) A total of 94 discernible DNA fragments were observed by 8 primers analysed,among which 82 fragments being polymorphic, The percentage of polymorphic was 87.23%. (2) The genetic diversity of the population in C.auratus in Qihe River was comparatively low calculated in POPGENE, PPB was 61.70%, H of 0.2036, I of 0.3085. (3) The inter-populations Nei’s genetic differentiation coefficient and gene flow were 0.1303 and 3.3359 respectively, indicating that the genetic differentiation among three populations had not reached the population level yet. (4) C.auratus in Jindihe River and C.auratus in Qinhe River stocks had the genetic distance relatively nearby, and C.aurutus in Qihe River stock had a degree of genetic differentiation and formed the genetic structure which was stable and independent.2. Genetic analysis of Cytb among C.auratus in Qihe River, eight C.auratus populations in wild and others near species of C.auratusMitochondrial DNA cytochrome b (Cytb) gene was amplified by PCR and sequenced bidirectional in C.auratus in Qihe River and eight C.auratus populations in wild. The results showed that there were no differences in sequences among the nine populations and the sequence was about 1140bp in full length. The Cytb gene sequence of C.auratus in Qihe River and the other six populations had no variation with the exception of C.auratus in Jiuli Lake and C.auratus in Datanggang Reservoir.The Cytb gene sequence of C.auratus in Qihe River contained 29.1%, 27.9%, 28.6%, 14.4% of T, C, A and G, respectively.The result of C.auratus in Qihe RiverA+T(57.7%) > G+C(42.3%) was similar to that in other seven populations with the exception of C.auratus in Jiuli Lake. The Cytb gene sequence of C.auratus in Qihe River showed that the bases had a obviously bias and had low content of G(14.4%), in the first position of codon only contained 9.3% and the third position of codon contained 14.0%, the second position basically did not have bias. The other eight populations also had the same features.The result of phylogenetic tree constructed by Neighbor-Joining method indicated that C.auratus in Qihe River had the closest phylogenetic relationship with the common C.auratus, had the second distance with C.auratus var. Pingxiangnensis, had far distance with C.gibelio in Japan, C.gibelio in Greece, C.langsdorfii in Japan and C.langsdorfii in Czech Republic, had the farest distance with C.carassius.

Related Dissertations

  1. Comparison of Genetic Diversity of Total Planktonic Bacteria and Active Planktonic Bacteria in Huguangyan Maar Lake in Summer,Q938
  2. Shiraia its separation of fungal genetic diversity analysis,S567.39
  3. Genetic Diversity Analyses of Sillago Sihama Using AFLP Marker,S917.4
  4. Growth Rate and Growth Model of the F5 Selected Lines for Four Shell Colors of Pinctada Martensii （Dunker） Cultured in Deepwater Area of Beibu Bay in Zhanjiang and Their Genetic Diversity Analysis Using SSR Molecular Markers,S968.31
  5. Pathogenic Differentiation and Genetic Diversity of Wheat Sharp Eyespot in Henan Province,S435.121
  6. ISSR Analysis of Genetic Diversity on 21 Lotus(Nelumbo Nucifera) Cultivars,S682.32
  7. Genetic Diversity and Ultrastructural Studies of Southern Corn Rust in China,S435.131.4
  8. SRAP and SSR molecular markers of pink flower Dendrobium genetic diversity and population genetic structure,S567.239
  9. The Research of Microsatellite Markers and the Eatablishment of Growth and Development Model in Xianan Cattle and Pinan Cattle,S823
  10. Molecular Characterisation Based on ITS and Genetic Diversity of the Heterodera Avenae Group on the Huang-huai Floodplain of China,S435.121
  11. Characterization of Population Structure and Linkage Disequilibrium of Chinese Soybean Landerace Population and QTL Association Analysis of Traits Related to Breeding for Soybeans,S565.1
  12. Genetic Diversity, Genetic Variance Association Mapping of Fresh Seed Quality Traits QTLs of G.Max in China,S565.1
  13. Studies on Genetic Diversity and Phylogenetic Relationship of Dendranthema and Its Related Genera,S682.11
  14. Polymorphisms of Y-STRs in Uygur and Kazak Ethnic in Xinjiang,R394
  15. The Research on Population Characters and Evaluated the Genetic Diversity of Chinese Wild Pyrus Ussuriensis Maxim.,S661.2
  16. The Genetic Diversity of 12 STR Loci in Four Dog Breeds and the Application in Individual Discrimination,S829.2
  17. Study on Diversity of CHS Gene from Buckwheat Germplasm Resources,S517
  18. Study on Genetic Diversity on ORFs Regions and Indica-Japonica Consanguinity Differentiation of Weedy Rice,S511
  19. Genetic Diversity in Different Populations of Tilapia (Oreochromis) by Microsatellite DNA Analysis,S917.4
  20. Genetic Diversity of Chimonanthus Praecox (L.) Link Based on ISSR Markers,S685.99
  21. AFLP Analysis on Genetic Diversity of Larix Principis-rupprechtii Mayr. In Shanxi,S791.22

CLC: > Agricultural Sciences > Aquaculture, fisheries > Aquatic basic science > Aquatic Biology > Aquatic Zoology
© 2012 www.DissertationTopic.Net  Mobile