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Role of a Two-Component Signal Transduction System 2148HK/RR in Pathogenicity of Streptococcus Suis Type 2

Author: ChengGong
Tutor: FanHongJie；TangJiaZuo
School: Nanjing Agricultural College
Course: Preventive Veterinary Medicine
Keywords: Streptococcus suis type 2 The binary signal transduction system Knockout Mutant Animal models
CLC: S858.28
Type: Master's thesis
Year: 2008
Downloads: 75
Quote: 4
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Abstract


Construction of Streptococcus suis type 2 (Streptococcus suis type 2) the virulent strain 05ZYH33 binary signal the transduction system 2148hk/rr gene knockout mutants. Build spectinomycin resistance gene in the middle, on both sides of the for 2148hk/rr coding genes downstream homologous sequences knockout plasmid screening 2148hk/rr encoding gene by homologous recombination knockout mutants. PCR analysis and Southern blot analysis showed 2148hk/rr encoding gene completely replaced by spectinomycin resistance gene knock-out in addition to successfully constructed mutants. Screening to get 05ZYH33 component signal transduction system 2148hk/rr gene knockout mutant △ 2148hk/rr laid the foundation to elucidate the regulatory system's role in the pathogenicity of Streptococcus suis. First select the different strains of mice to assess the susceptibility of 05ZYH33. 10 each of 28-day-old BALB / c and ICR strains of mice by intraperitoneal inoculation bacteria liquid per 1mL (approximately 5 × 10 8 CFU). Inoculation 14 days, BALB / c mice group full death 6 ICR mice group survival, suggesting more susceptible BALB / c mice 05ZYH33, can be used for assessment of pathogen virulence. To determine 05ZYH33 median lethal dose in BALB / c mice (LD 50 ) 30 BALB / c mice were randomly divided into 6 groups, 1-5 groups respectively using different dilutions of bacterial suspension The intraperitoneal inoculation, Section 6 THS of the injection of the same volume of culture medium as a negative control. The results showed, LD 50 8.4 × 10 7 of CFU. The test lay the foundation for the in-depth study of the pathogenesis of Streptococcus suis. Using two animal models of mice and piglets assessment component signal transduction system in the pathogenic process 05ZYH33 2148hk/rr. In a mouse model, 30 of the 28-day-old BALB / c mice were randomly divided into three groups. Group 1 by intraperitoneal inoculation 05ZYH33 diluted broth per 1mL of about 5 × 10 8 CFU bacteria; group 2 in the same manner inoculated △ 2148hk/rr diluted broth; three groups injected with the same volume THS as negative controls. Virulent mice all died within two days, the mutant mice mild disease, no deaths occurred. Piglet model, the nine-week-old piglets were randomly divided into three groups 1 and 2 were intravenously inoculated with 1mL (about 10 8 CFU) of the wild strain and mutant bacterium; No. 3 group was injected with an equal volume of PBS as a negative control. 2 days after inoculation, the wild strain and mutant strains of two groups of piglets of all deaths, and none of the control group piglets incidence. Animal the test prompts 2148hk/rr Streptococcus suis pathogenic effect of different host animals there are significant differences in the regulatory system in Streptococcus suis type 2 infection pathogenic process is worth exploring.

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CLC: > Agricultural Sciences > Livestock, animal medicine,hunting,silkworm,bee > Animal Medicine ( Veterinary Medicine) > Livestock, poultry, wildlife diseases > Livestock > Pig
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