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Induction of Apoptosis by d-limonene is Mediated by Mitochondrial Death Pathway in Human Leukemia Cells

Author: JiJun
Tutor: DiaoFengSheng
School: Dalian Medical University
Course: In Integrative Medicine clinic
Keywords: d-limonene leukemia apoptosis mitochondrial pathway
CLC: R733.7
Type: Master's thesis
Year: 2011
Downloads: 3
Quote: 0
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Abstract


Objective: To investigate the apoptosis effect induced by d-limonene in human human leukemia cells and its mechanism.Methods: Using K562 and HL-60 cell lines, we investigated the anti-tumor activity of d-limonene. Apoptosis was evaluated by Annexin V/PI binding assay. Flow cytometry and western blot were performed to evaluate expression of some apoptosis-regulating proteins.Results: d-Limonene induced apoptosis in a dose- and time-dependent manner in both tested cells. We found an increase in Bax protein expression, the release of cytochrome c from mitochondria, and an increase in caspase-9 and cleaved caspase-3, but not caspase-8. Broad-spectrum caspase inhibitor and a caspase-3-specific inhibitor were able to prevent activation of d-limonene induced apoptotic cell death.Conclusions: The caspase-dependent mitochondrial death pathway is primarily involved in the development of d-limonene-induced apoptosis in human leukemia cells.

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CLC: > Medicine, health > Oncology > Hematopoietic and lymphoid neoplasms > Leukemia
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