Dissertation > Excellent graduate degree dissertation topics show

Effect of Chloride Channel Blocker on the Hippocampal Neuronal Apoptosis Induced by No in Culture

Author: YinJinBao
Tutor: ChangQuanZhong
School: Zunyi Medical College,
Course: Physiology
Keywords: PARP-1 SIN-1 hippocampal neurons Apoptosis AIF
CLC: R363
Type: Master's thesis
Year: 2011
Downloads: 4
Quote: 0
Read: Download Dissertation

Abstract


Ojective:To investigate the protective effects of DIDS on apoptosis of cultured hippocampal neurons induced by SIN-1 (a donor of NO) and the effect of DIDS on expression of PARP-1 and AIRMethods:The primary cultured neurons of 12days old from SD rats wre randomly divided into three groups:control groups、SIN grops and SIN-1+DIDS grops.The duration of both SIN-1 and DIDS effect on neurons for 18h.The neuronal viability was tested by MTT assay.Fluorescent staining with Hoechst33258 was used to analyze apoptosis.The methods of Western blot and immunocytochemical fluorescent stain were used to detect the expression of PARP-1 and AIF.Results:(1)SIN-1 may induced cultured hippocampal neuronal apoptosis with dose-dependence and 1.0mmol/L of SIN-1 can induce neuronal apoptosis obviously. (2)The chloride channel blockers of DIDS could inhance the neuronal viabilities and inhibitted the neuronal apoptosis induced by SIN-1.(3)Protein PARP-1 and AIF increased expression after the SIN-1 induce neuronal apoptosis.(4)Chloride channel blockers DIDS could significantly reduce the expression of PARP-1 and AIF induced by SIN-1.Conclusion:Chloride channel blocker DIDS perheps can save injured neuron by the way of influence PARP-1/AIF in which of non-caspase dependent aopotosis pathway.

Related Dissertations

  1. The Study on Cryopreservation and Mechanism of Freezing Injury on the Spermatozoa of Coelomactra Antiquate,S968.3
  2. The Anti-tumor Effect of CADPE and Inducing Apoptosis in Human Gastric Cancer Cells,R735.2
  3. Cloth and β- elemene combined administration of anti-tumor effect and mechanism of celecoxib,R96
  4. TRAIL in the regulation of tumor invasion CD4 ~ CD25 ~ Treg,R730.2
  5. The Study of Lead Exposure on Cyprinus Carpio Ovary Epithelial Cells,X174
  6. Effects of Diclazuril on G3PDH in Second-generation Merozoites of Eimeria Tenella,S858.31
  7. The Effects and Preliminary Study of PCV2 on Ca2+ Signal in Lymphocytes of Piglets,S858.28
  8. Effects of Angiogenic Factor on Follicular Angiogenesis and Development in Sexual Maturity Mice,S852.2
  9. Expression of β-Catenin in Pig’s Ovary and the Effect of β-Catenin on Porcine Granulosa Cells Apotosis and Steroidogenesis Related Enzyme,S828
  10. Induced Combination Apoptosis by FB1 and AFB1 in Vero Cell,S856.9
  11. The Apoptosis Mechanism Induced by Aflatoxin B1 and Deoxynivalenol in Primary Hepatocyte of Cyprinus Carpio,S856.9
  12. Effects of BMPR-IB Gene Silencing by Small Interfering RNA on Apoptosis of Porcine Pollicular Granulosa Cells and Ecpression of BMP Pathy-Way-Ralted Genes,S828
  13. The Effects of Don on Proliferation, Differentiation, and Apoptosis of Chondrocytes in Chicken,S858.31
  14. The Effect of Foxol on Apoptosis of Mouse Granule Cells,S865.13
  15. Study of Melittin on Tumor Inhibition and Part of Mechanism of Human Hepatocellular Carcinoma HepG-2 Xenograft in Nude Mice,R735.7
  16. The Protective Effect of Rat Myocardial Ischemia/reperfusion Injury Following Bone Marrow Mesenchymal Stem Cell Pretransplantation for 1 Week,R542.22
  17. Expression of WW Domain Containing Oxidoreductase Gene in Cholangiocarcinoma and Its Effect on the Biological Behavior of Cancer Cell Line QBC939,R735.8
  18. The Effects of (180F-FDG on the Apoptosis of Eca-109 Esophageal Cancer Cell Line,R735.1
  19. The Signal Mechanism Underlying DON Induced Apoptosis in BHK-21 Cells,S856.9
  20. The Expression and Mechanism of NF-κ B and IL-6 after 90% Portal Vein Ligation in Rats,R657.3
  21. The Study of Mitochondrial Signal Mechanism Induced Apoptosis by Don in Human Colon Cancer Cells,R735.35

CLC: > Medicine, health > Basic Medical > Pathology > Pathophysiology
© 2012 www.DissertationTopic.Net  Mobile