Dissertation > Excellent graduate degree dissertation topics show

The Empirical Study of Bone Marrow Mesenchymal Stem Cells are Induced Osteoblasts by Alendronate and Icariin

Author: ShuZhiYong
Tutor: ChaZhenGang
School: Jinan University
Course: Surgery
Keywords: Alendronate Icariin bone marrow mesenchymal stem cell Osteoporosis Osteogenic differentiation
CLC: R285.5
Type: Master's thesis
Year: 2009
Downloads: 90
Quote: 0
Read: Download Dissertation

Abstract


Objective:To research the influence of ALN and ICA on the ability to proliferate and to differentiate into osteoblast of Bone mesenchymal stem cells（BMSCs）,observe the the influence of ALN and ICA on the expression of transforming growth factor-betal（TGF-β1） and bone morphogenetic protein-2（BMP-2） in the progress to promote MSCs differentiate into osteoblast and analyze the appoach of ALN and ICA to go into effect can provide experimental evidence to explain the mechanism of ALN and ICA in preventing and treating OP,feasible experimental exploration in establishing a platform of TGF-β1 and BMP-2 to screen the medicine of preventing and treating OP and a new thought of preventing and treating OP by traditional Chinese and western medicine.Methods:1.BMSCs from rabbits were isolated and fostered by using differential time adherent method method;2.To observe the growth,proliferate and morphologic characteristics of the original cells and their descendant by inverted phase contrast microscope day by day and darw their auxodrome.3.To get the well growing cells of third generation,and identify BMSCs by detecting surface markers and cell life cycle by morphology and flow cytometer;4.MTT method was used to confirm the most effective concentration of the ALN and ICA on the proliferation of BMSCs;5.The most effective concentration of the ALN and ICA on the differentiation of BMSCs into osteoblast was jurged by alkaline phosphatase（ALP）;6.According to the different induced condition,MSCs were divided into 3 groups: control group,classic group（induced by the classic osteoblast-induced system） and drugs group（induced by the most effective concentration of drugs on differentiation into osteoblast）.ALP and calcium nodes in each group were detected to indicate the osteoblast-formation function of different group; 7.ELISA was used to detect the expression of TGF-β1、BMP-2 of each induced group in the progress of BMSCs differenting into osteoblast to explain the mechanism of the drugs in inducing BMSCs differenting into osteoblast;8.To reach the conclusion by contrasting and analyzing the statistics methods.Result:1.The results of the surface antigens of BMSCs detected by flow cytometer showed the positive expressions of CD29 and CD44 and the negative expressions of CD34 and CD45;MSCs isolated from rabbit could be induced successfully into osteoblasts,chondrocytes and adipocytes;2.The most effective concentration of the ALN and ICA on the proliferation of BMSCs were 1×10-8mol/L and 1×10-9mol/L;and the most effective concentration of them on the differentiations of BMSCs into osteoblast were 1×10-8 mol/L and 1×10-9mol/L;the ALN(1×10-8mol/L) and ICA(1×10-9mol/L) could both increase the expression of osteoblast-indices which follwed the increase of the secretion of TGF-β1 and BMP-2;3.ALN and ICA which had been proved to possess the ability to induce BMSCs differenting into osteoblast in former study could both increase the expression of TGF-β1 and BMP-2.Conelution:1.The stable and uniformal BMSCs of rabbit could be obtained by differential time adherent method;2.The ALN and ICA could both promote the proliferation of the BMSCs and the differentiation of BMSCs into osteoblast;3.In the osteoblast-differentiati on progress,the induced groups of the drugs in this expriment all could increase the expression of TGF-β1 and BMP-2,which may be one of the mechanisms that could promote the differentiation of BMSC into osteoblast.

Related Dissertations

  1. Chronic obstructive pulmonary disease osteoporosis and TCM kidney deficiency syndrome research,R259
  2. Study of Correlation between Serum Adiponectin Level and Arteriosteogenesis in Patients with Osteoporosis,R580
  3. Study on Cloning and Expression of Active Domain of Rankl and Its Antiserum Preparation in Chicken,S831
  4. The Study of Rabbit Bone Marrow Mesenchymal Stem Cells Separation Culture and Directional Differentiation,R329
  5. Comparative Efficacy with Locking Plate and the Normal Plate for Treatment of Elderly Tibial Plateau Fractures in Schatzker Ⅱ, Ⅲ Type,R687.3
  6. The Curative Effect Contrast between the Percutaneous Hollow Compression Screw Internal Fixation and the Prosthetic Replacement for Joint in the Senile Osteoporosis Femoral Neck Fracture,R687.3
  7. Preliminary Study on Angiogenesis in Ischemic Hindlimb of Diabetic Rat Model after Mesenchymal Stem Cell Implantation,R587.1
  8. Differentiation Capacity of Periodontal Ligament Stem Cells of Chronic Periodontitis Patients with and Without Type 2 Diabetes Mellitus,R587.1
  9. The Beneficial Effects of Soy Isoflavones in Osteoporosis of Ovariectomized Rats,R580
  10. Effect of Advanced Glycation End Products (AGEs) on Biological Characteristics of Lipopolysaccharide (LPS)-mediated Periodontal Ligament Stem Cells,R781.4
  11. Exercise, fresh milk and calcium for osteoporosis in older women Mongolian populations affected,R580
  12. The Influence of Exogenous Hyaluronic Acid on Chondrogenic Differentiation of Rabbit Bone Marrow Mesenchymal Stem Cells,R329
  13. The Value of Proximal Femoral Geometric Structure and Bone Mineral Density in Evaluating the Risk of Hip Osteoporotic Fracture,R683
  14. Vertebroplasty Versus Nonoperative in Treatment of Vertebral Compression Fractures: A Systematic Review,R687.3
  15. PVP and PKP Treatment of Osteoporotic Thoracolumbar Vertebral Compression Fracture Analysis of the Efficacy,R687.3
  16. Clinical Observation of Percutaneous Vertebroplasty in the Treatment of Thorocolumbar Osteoporotic Vertebral Compression Fractures,R687.3
  17. Observation of Percutaneous Kyphoplasty for Osteoporosis Vertebral Compression Fracture with High Viscosity Bone Cement,R687.3
  18. The Clinical Research on Blood Counts in the Patients with Osteoporosis,R580
  19. The Observation of Curative Effect of Different Medicines in Treating Postmenopausal Osteoporosis and the Evaluation of the Quality of Life and Pharmacoeconomics,R580
  20. Serum 25-hydroxy Vitamin D Level in Diabetic Patients and Its Effect on Blood Glucose and Bone Mass,R587.1
  21. The Influence of Exercise on OPG and RANKL Expression of Ovariectomized Rats,R580

CLC: > Medicine, health > Chinese Medicine > Of Pharmacy > Pharmacology > Chinese medicine Experimental Pharmacology
© 2012 www.DissertationTopic.Net  Mobile