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Effects of 17α-estradiol on the Aβ Synthesis of APP/PS1 N2a Gene Transgenic Cells

Author: ZengJie
Tutor: LeiDeLiang
School: Central South University
Course: Neurobiology
Keywords: 17α-estradiol Neuro-2α APP/PS1 β-Amyloid
CLC: R739.4
Type: Master's thesis
Year: 2010
Downloads: 2
Quote: 0
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Abstract


Objective To investigate the effects of 17a-estradiol (E2a) on the release ofβ-amyloid protein (Aβ) with the transgenic cell line APP/PS 1 N2a, and discuss the potential mechanism of such effects.Methods APP/PS 1 N2a cell lines were pretreated with 10 nM E2a for 2 days, then changed the culture medium, and treated with different methods:(1) cell lines were treated with either E2a (25nM,50nM, 100nM,200nM) or vehicle, The protein level of Aβand BACE1 both intracellular and extracellular of APP/PS 1 N2a cells were detected by Western blot. (2) cell lines were treated with 100nM E2a,20 mM glucose and 5 mU glucose oxidase(GOX), followed by exposure to the glucose oxidase (G/Gox) to induce oxidative stress. After 24 hours, the change of the ROS contents in APP/PS 1 N2a cells was observed for DHE.Results Pretreatment of APP/PS 1 N2a cells with different concentration (25nM,50nM, 100nM,200nM) E2a significantly reduced extracellular levels of AP(P<0.05). There was no change both in the intracellular levels of Aβand the levels of BACE1(P>0.05). E2a can reduce the ROS produced by APP/PS 1 N2a cells after G/Gox treatment.Conclusion E2a can reduce the production of extracellular Aβ,its mechanism may be related to the oxidation damage.

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