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Research on Biological Effects of Multi-scale Titanium Dioxide Nano-particles

Author: YangFuGuang
Tutor: YangYongZuo
School: Second Military Medical University
Course: Biophysics
Keywords: Titanium Dioxide Nanoparticles Ultrastructure Fluorescent Labelling Transmission Electron Microscope Biological Effect
CLC: TB383.1
Type: Master's thesis
Year: 2009
Downloads: 180
Quote: 0
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Abstract


Nano-titanium dioxide （Titanium Dioxide,TiO2） is widely used in paints,paper coatings,plastics,elastomers,cosmetics,sunscreen and other supplies.Application of nano-titanium dioxide has been extended to other areas,such as ultrafine particles, electronic materials,denitriding catalyst,photoelectrocatalyst and so on.Therefore,the nano-TiO2 shows immense market prospects,and has been thought highly of at home and abroad.TiO2 has been considered as hypotoxic dust in the past,in many toxicological studies about dust,TiO2 is often used as a non-toxic dust control.However,the studies find that ultrafine TiO2 （average diameter of 60nm）-induced rat lung inflammation is even more serious than micro-TiO2 （average diameter of 250nm） which has the same air quality concentrations.The report has changed people’s awareness of the problem of particle toxicity,even non-toxic or hypotoxic fine particulate material,its ultrafine particles may also become toxic.Because of the unique physical and chemical properties of TiO2 nanoparticles,it is difficult to use conventional methods and means of detection.After nano-TiO2 entering the cells,the scavenging capacity of cells will decrease as the size of the TiO2 particles shrinks,and it will result in the damage of cell membrane,changes of cell ultrastructure,fracture of nuclear DNA,and changes of protein content and enzyme activity,so it is necessary to study its biological effects and toxic effects.In this study,fine different scales of TiO2 nanoparticles and TiO2 microparticles are selected as research objects which are widely applied.Human keratinocytes （HaCaT）,pulmonary epithelial cells （A549） and mouse monocyte macrophages （RAW） are used as the model.This research has explored the biological effects of TiO2 nanoparticles from the conditions of depolymerization,whether entering the cells or not,the way of entering cells,the damage to cells,and the impact on DNA synthesis.According to the unique nature of nanoparticles,the influence of nano-TiO2 on cell ultrastructure in vitio,biochemical indicator and pathological changes are studied systematically in this article.The dose effect, time effect and size effect between nano-TiO2 and cells are also discussed in detail.This article has still made a further study about the influence of TiO2 nanoparticles on DNA synthesis,and has established and developed test methods and experimental techniques about biological effects and toxicology of nano-matericals.The results are as follows: 1. The depolymerization and dispersion of five different scales of TiO2 nanoparticles and TiO2 microparticles show good under the conditions of the power at 300W,the temperature around 25℃and the ultrasound about 30min .2. Five different scales of TiO2 nanoparticles and TiO2 microparticles can enter cells,and can maintain a good dispersibility and stability after entering. The volume of TiO2 nanoparticles in the cells is related to its concentration, scale and the time that it enters cells.3. Compared to silicon dioxide （SiO2） and four three iron oxide （Fe3O4） nanoparticles,TiO2 nanoparticles mainly enter cells by way of phagocytosis, combining the may of the direct rupture of membrane,while the SiO2 nanoparticles enter cells by the two ways .4. The damage of TiO2 nanoparticles and TiO2 microparticles on the cell ultrastructure was related to its dose .5. The impact of TiO2 nanoparticles on cytoskeletal filament shows a dose– effect Relationship at a certain concentration range.6. TiO2 nanoparticles caused cells to produce reactive oxygen species and made n the cells oxidative damage. MDA content which TiO2 nanoparticles induced cells to produce and TiO2 nano-particle concentration shows a dose - response relationship.7. The impact of TiO2 nanoparticles and TiO2 microparticles on cell viability shows a dose-effect relationship, but its size there were no significant differences.8. The inhibiton of TiO2 nanoparticles on the DNA synthesis is closely related to its dose and particle size.

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