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The Therapeutic Effect of Recombinant Rat Interferon-gamma Against Rat with Severe Aeute Panereatitis

Author: LiYong
Tutor: LuoHuaYou；ChengRuoChuan
School: Kunming Medical College
Course: General Surgery
Keywords: recombinant rat interferon-gamma severe acute pancreatitis MHC-Ⅱ cytokines
CLC: R96
Type: Master's thesis
Year: 2009
Downloads: 36
Quote: 0
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Abstract


Objective:Severe acute pancreatitis(SAP) is a severe acute abdomen surgery emergency,which often occurs incidently and is associated with substantial alterations of the immunologic host response which has been claimed to promote multi organ dysfunction syndrome(MODS) and high mortality rate.The pathogenesy is not elucidated quite well until now.Treatment with immuno-modulating substances has been subject of few experimental studies with still conflicting results.This study is to investigate whether recombinant rat interferon-gamma(rrIFN-γ) has the protective effect in rats with SAP and investigate the possible mechanism of severe acute pancreatitis.Methods:Ninety-six male Spragwe-Dawley rats were randomly divided into three groups:sham-operation(SO) group(n=32),SAP model group(n=32) and treatment with rrIFN-γgroup(n=32).The amount changes of ascites,pancreatic macroscopic scores,pancreatic histologic scores and serum level of amylase,lipase,TNF-αand IL-10 were examined at 1.5h,3h,6h,12h time point.The expression of MHC-ⅡmRNA were detected by semi-quantitative reverse transcription polymerase chain reaction(RT-PCR).Results:The amount of ascites decreased significantly in treatment group compared with SAP group at 1.5h,6h,12h(P＜0.05).The pancreatic macroscopic scores decreased significantly in treatment group compared with SAP group at 3h,6h,12h (P＜0.05 or P＜0.01),which still raise obviously in treatment group compared with SO group(P＜0.01).The pancreatic histologic scores decreased significantly in treatment group compared with SAP group at 4 time ponits(P＜0.05 or P＜0.01),which still raise obviously in treatment group compared with SO group at 4 time ponits(P＜0.01).The serum level of amylase decreased significantly in treatment group compared with SAP group at 4 time ponits(P＜0.01).The serum level of lipase decreased significantly in treatment group compared with SAP group(P＜0.05 or P＜0.01).The serum level of TNF-αdecreased significantly in treatment group compared with SAP group at 3h, 6h,12h(P＜0.05 or P＜0.01),which still raise obviously in treatment group compared with SO group at 4 time ponits(P＜0.05).The serum level of IL-10 raise significantly in treatment group compared with SAP group(P＜0.05) and SO group(P＜0.01) at 4 time ponits.The MHC-ⅡmRNA expressed in SO group which had a higher level compared to SAP group at 3h,6h,12h(P＜0.01).The pancrea of SO group can express MHC-ⅡmRNA,which raised significantly compared with SAP group(P＜0.01).The level of MHC-ⅡmRNA expression released significantly in treatment group compared with SAP group(P＜0.01) and SO group at 3h,6h,12h(P＜0.01).Conclusion:Severe acute pancreatitis is associated with early alterations of the immune suppressive response comprising overt low expression of MHC-ⅡmRNA in pancrea.Targeting immunologic derangement with recombinant rat interferon-gamma (rrIFN-γ) improves local pancreatic damage and systemic severity of rats with SAP.

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