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Preliminary Study of Apoptotic Inhibition and Its Molecular Mechanism of Dexamethasone on Cisplatin-induced Human Lung Adenocarcinoma Cell SPC-A1

Author: CaoFei
Tutor: ZhangZhiHui
School: Guangxi Medical University
Course: Oncology
Keywords: Dexamethasone Glucocorticoids receptor Lung cancer Chemotherapy Anti-apoptosis
CLC: R734.2
Type: Master's thesis
Year: 2009
Downloads: 12
Quote: 0
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Abstract


Objective:To investigate the inhibition efficacy and molecular mechanism of dexamethasone(DEX) on apoptosis of human lung adenocarcinoma cell SPC-A1 induced by cisplatin(CDDP).Methods:SPC-A1 cells was pre-cultured in vitro for 24 hours with DEX in different concentrations and then CDDP was added and the cells were cultured for another 48 hours,then the survival rates of the cells determined by MTT.SPC-A1 cells were stimulated by 1μmol/L DEX,and then total RNA were extracted respectively at 1hr,2hr, 4hr,6hr,12hr.The expression of the serum/glucocorticoid-induced kinase(SGK-1) and mitogen-activated protein kinase phosphatase-1(MKP-1) in SPC-A1 cells was detected by semi-quantitative RT-PCR technology.The expression of glucocorticoid receptor(GR) in SPC-A1 cells was measured by immunohistochemistry(IHC) with antibody of biotin-labeled anti-GR.The results:SPC-A1 cells showed resistance to CDDP-induced apoptosis when pre-cultured DEX,and the resistance intensity was DEX concentration-dependent.The SPC-A1 cells stimulated by DEX expressed SGK-1 which peak level occurred at 12hr. Up-regulated expression of GR in SPC-A1 cells stimulated by DEX was detected by IHC.The number cells expressing GR in SPC-A1 cells was significantly higher than that the control group.But the expression of MKP-1 was not detected Conclusion:DEX inhibits apoptosis of SPC-A1 cells induced by CDDP.The possible molecular mechanism is that increased expression of GR up-regulates the expression of SGK-1,which locates at the downstream of anti-apoptosis pathway,and the apoptosis of resistance.SPC-A1 cells may account for all above the factors.

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CLC: > Medicine, health > Oncology > Respiratory system tumors > Lung tumors
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