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IL-24 Inhibit the Growth and Induce the Apoptosis in Cervical Cancer Hela

Author: CaoTao
Tutor: LiLi
School: Shanxi Medical
Course: Obstetrics and Gynaecology
Keywords: IL-24 Cervical cancer cell line Hela proliferation apoptosis
CLC: R737.33
Type: Master's thesis
Year: 2009
Downloads: 54
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Abstract


Objective:To study the effect of tumor suppressor gene IL-24 on the cell growth and apoptosis in cervical cancer cell line Hela.Methods:The shuttle vector pAd track CMV that carried the objective gene of IL-24 was transfected into cervical cancer cell line Hela. Methyl thiazolyl tetrazolium (MTT) rapid photocolorimetric assay was used to examine the cell proliferation of Hela. Flow cytometry was used to examine the cell cycle and rate of apoptosis of Hela.At the same time, pAd track CMV were transfected into Hela as empty-vector control. wild-grow Hela as negative control.Results:1.As showed by MTT assay, the OD values of three groups have a total diffference. (P<0.001). Compare to the other two groups, the OD values of IL-24 group was obviously reduced (P<0.001), but no significant difference was shown between empty-vector group and negative control group(P=0.100).2.As indicated by flow cytometry, the apoptosis rates of three groups have a total difference. (P<0.05). Compare to the other two groups, the apoptosis rates of IL-24 group was obviously reduced(P<0.05), but no significant difference was shown between empty-vector group and negative control group(P>0.05). In addition, compare to the other two groups, the number of cells in G0/G1 period was increased and cells in S period was reduced (P<0.05), but no significant difference was shown between empty-vector group and negative control group(P>0.05).Conclusion:1. IL-24 gene can inhibit the growth of cervical cancer cell line Hela.2. IL-24 gene can induce the apoptosis of cervical cancer cell line Hela.

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CLC: > Medicine, health > Oncology > Genitourinary tumors > Female genital tumors > Uterine tumors
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