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The Expressions and Clinical Significance of p53、Survivin and Cycooxygenase2(COX-2) in Hydatidiform Mole by IHC

Author: ChenLingZhi
Tutor: ZuoXiaoJuan
School: Hebei Medical University
Course: Obstetrics and Gynaecology
Keywords: p53 Survivin COX-2 Mole Immunohistochemistry
CLC: R737.33
Type: Master's thesis
Year: 2009
Downloads: 45
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Abstract


Objective: gestational trophoblastic disease is a group derived from placental trophoblast cell disease, including hydatidiform mole, invasive mole, choriocarcinoma (choriocarcinoma) and placental site trophoblastic tumor. Trophoblastic implantation, placenta formation process of a special epithelial cells, placental trophoblast cells have similar characteristics of malignant tumors, the performance for the rapid proliferation and erosion of the endometrium, but its erosion with strict time and space restriction, It is the biggest difference to nourish the cells and malignant cells. Mole is gestational trophoblastic disease the most common type, mole cause is not yet clear trophoblast cells compared to normal pregnancy, hydatidiform mole trophoblastic myelodysplastic active properties, hydatidiform mole, although benign disease , but there are certain malignant tendency, no early prediction of gestational trophoblastic disease. Generally agreed that the malignant tendency of patients with hydatidiform mole may be given preventive chemotherapy, but preventive chemotherapy should not be routinely used in all patients with hydatidiform mole. P53 gene is an important tumor suppressor gene, it is mainly to protect cell DNA from damage by cell cycle arrest and induction of apoptosis; Survivin is a member of the inhibitor of apoptosis family (IAP), studies have shown that Survivin in apoptosis and cell cycle gene regulation play an important role; cyclooxygenase -2 (cyclooxygenase-2, COX-2) is the rate-limiting enzyme of prostaglandin biosynthesis, COX-2 up-regulation is an important rate-limiting in tumorigenesis step of COX-2 may promote tumor cell proliferation and inhibition of apoptosis and promote tumor malignant transformation, or by promoting tumor angiogenesis mechanisms involved in tumor development. This issue through the detection of p53, Survivin and COX-2 in normal pregnancy villi, molar tissue expression and its correlation to explore the three occurred in the mole, the development of the role, for the prediction of gestational trophoblastic theoretical basis. Were collected the 2000-2006 Third Hospital of Hebei Medical University first inpatient curettage molar tissue was obtained from 41 patients, average age 31.5 years, a two-year follow-up, 29 cases of malignant transformation is called the mole is not malignant group, 12 cases of malignant hydatidiform mole is called the gestational trophoblastic group; 17 cases of normal pregnancy fluff control tissue taken from the 2006 family planning clinics in the Third Hospital of Hebei Medical University, pregnant patients with normal pregnancy, the average age was 27.5 years. By immunohistochemistry (immunohistochemistry) PV-6000 detection of p53, Survivin and COX-2 protein expression in all samples, and to explore the correlation of p53 and survivin, COX-2 between. All the results statistically application SPSS12.0 statistical software for analysis. Result 1.p53 protein expression in all specimens positive staining in the nucleus. p53 in normal chorionic villi, the mole is not malignant group, the gestational trophoblastic group expressed positive rates were 11.76%, 48.28%, 91.67%; there is a significant difference among the three groups (x2 = 18.12, P lt; 0.01) . Normal chorionic villi, the mole is not malignant group and gestational trophoblastic group of pairwise comparison of the level of p53 protein expression difference were statistically significant (P lt; 0.05, P lt; 0.01, P lt; 0.01) Survivin protein expression in all specimens positive staining located in the to nourish the nucleus and cytoplasm, but mainly localized in the cytoplasm. Survivin in normal villi have a certain positive expression rate was 23.52%, and the mole is not malignant group and the the gestational trophoblastic group expressed positive rates were 41.38%, 75.00%. Differences exist between the three groups was statistically significant (x2 = 7.67, P lt; 0.05) Mole is not malignant and normal chorionic villi group difference was not statistically significant (P gt; 0.05); the mole without malignant group and gestational trophoblastic group difference was not statistically significant (P gt; 0.05); gestational trophoblastic disease group normal chorionic villi of Survivin expression level was a significant difference (P lt; 0.01) COX-2 expression in all specimens positive staining in the nourishing cytoplasm. COX-2 in normal chorionic villi, the mole is not malignant group the gestational trophoblastic group expressed positive rates were 35.29%, 58.62%, 91.67%; there is a significant difference between the three groups (x2 = 9.22, P lt; 0.05 ). COX-2 mole is not malignant group and with gestational trophoblastic cells progression of the disease positive rate was gradually increased, but the mole is not malignant and normal chorionic villi group COX-2 protein expression level was no significant difference (P GT; 0.05); gestational trophoblastic disease group, the difference was not statistically significant (P gt; 0.05); comparison the gestational trophoblastic group with normal chorionic villi group, the difference was statistically significant (P lt; 01). In p53 positive and p53-negative group molar tissue of Survivin of the positive expression rate were 64.00% (16/25), 31.25% (5/16); COX-2, the positive expression rate was 72.00% (18/25) and 62.50% (10/16). Survivin expression of p53 positive group were significantly higher than the p53-negative group, the difference was significant (x2 = 4.19, P lt; 0.05), Spearman rank correlation analysis showed that the two were positively correlated (r = 0.362, P lt; 0.05); COX-2 expression positive rate of expression between p53 positive group and p53-negative group difference was not statistically significant (x2 = 0.41, P gt; 0.05) Spearman rank correlation analysis showed that p53 and COX-2 expression in all molar tissue no correlation (r = 0.297, P gt; 0.05) The conclusion 1.p53 protein expression may promote to nourish cell proliferation, associated with malignant mole; moderate Survivin is completed correctly embryonic cell mitosis and cell proliferation necessary condition Survivin the overexpression induced apoptosis block, may play an important role in gestational trophoblastic cells in the development of the disease process; COX-2's increased participation in gestational trophoblastic disease, probably by mechanisms development. 2.p53 expression of Survivin in the molar tissue was positively related to the upregulation of p53, Survivin expression may be through some kind of mechanism to make to nourish cell proliferation and apoptosis imbalance, participate in gestational trophoblastic cells in the development of the disease process and its mechanisms remain to be further explored.

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CLC: > Medicine, health > Oncology > Genitourinary tumors > Female genital tumors > Uterine tumors
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