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Objective: Vascular endothelial growth factor (VEGF), also known as vascular permeability factor, which can specifically promote endothelial cell division, proliferation, increased vascular permeability, promoting blood vessel formation; matrix metalloproteinases (MMPs) are a highly zinc +-dependent family of proteolytic enzymes, it can cause basement membrane digestion, cell migration, the formation of a new basement membrane, to promote the formation of new blood vessels. Both in tumor occurrence, development and metastasis process plays a very important role. Study found that the presence of VEGF and MMPs gene in some of polymorphic loci can regulate gene transcription or protein expression levels affect tumor occurrence and development. This study aimed to investigate the VEGF-2578C / A and VEGF 936C / T and MMP-12-82A / G and MMP-13-77A / G single nucleotide polymorphisms (single nucleotide polymorphism, SNPs) in northern China women with epithelial the relationship between the risk of ovarian cancer, from molecular biology to the prevention, diagnosis and treatment of ovarian cancer provide evidence. Methods: A hospital-based case - control study in 256 patients with ovarian cancer and 329 cases of healthy control women for the study. Using proteinase K-salting method to extract genomic DNA, polymerase chain reaction - restriction fragment length polymorphism (polymerase chain reaction-restriction fragment length polymorphism, PCR-RFLP) analysis method, the VEGF-2578C / A, VEGF 936C / T, MMP-12-82A / G and MMP-13-77A / G 4 SNPs were genotyped. Statistical analysis using SPSS11.5 software package (SPSS Company, Chicago, Illinois, USA) for processing. Normal control group genotype frequencies χ2 test done Hardy-Weinberg equilibrium analysis. Case group and control group, age differences using t test, genotype and allele frequencies were compared using χ2 test to non-conditional logistic regression method to calculate relative risk indicates the odds ratio (odds ratio, OR) and its 95% confidence interval (confidence interval, CI). EH software analysis using VEGF-2578C / A and VEGF 936C / T and MMP-12-82A / G and MMP-13-77A / G haplotype frequencies, 2LD software analysis linkage disequilibrium. P lt; 0.05 as statistically significant difference in standards. Results: 1 VEGF-2578C / A and VEGF 936C / T polymorphism and epithelial ovarian relationship 1.1 VEGF-2578C / A polymorphism genotype and allele frequencies in epithelial ovarian cancer group and the control group had no significant difference (P = 0.42 and P = 0.64). With A / AC / A genotypes, C / C genotype and epithelial ovarian cancer risk nothing, OR is 0.87 (95% CI = 0.63 ~ 1.21). In pathological type, clinical stage or age at diagnosis stratified analysis, VEGF-2578C / A genotype and allele frequencies distribution among the groups were not found significant differences (P values ??were greater than .05). 1.2 VEGF 936C / T polymorphism genotype and allele frequencies in epithelial ovarian cancer group and the control group there was no significant difference (P = 0.67 and P = 0.79). And T / TC / T genotypes, C / C genotype and epithelial ovarian cancer risk nothing, OR is 0.93 (95% CI = 0.65 ~ 1.32). In pathological type, clinical stage or age at diagnosis stratified analysis, VEGF 936C / T genotype and allele frequencies distribution among the groups were not found significant differences (P values ??were greater than .05). 1.3 using the EH and 2LD software analysis found VEGF-2578C / A and 936C / T SNPs do not exist between linkage disequilibrium (D '= 0.15). 2 MMP-12-82A / G and MMP-13-77A / G polymorphism and epithelial ovarian cancer between 2.1 MMP-12 -82 A / G SNP of A / A, A / G, G / G three genes type frequency in ovarian cancer group and the control group were 93.0%, 7.0%, 0 and 96.7%, 3.3%, 0, there is significant difference between two groups (P = 0.04); ovarian cancer group G allele significantly higher in both groups were significantly different compared (P = 0.04). With A / A genotype, A / G genotype significantly increased the incidence of epithelial ovarian cancer risk (OR = 2.19,95% CI = 1.01 ~ 4.72). Stratified according to age at diagnosis, with the A / A genotype, A / G genotype significantly increased lt; 50 years the incidence of epithelial ovarian cancer risk (OR = 3.00,95% CI = 1.19 ~ 7.36); According to the pathological type and clinical stage stratified with A / A genotype, A / G genotype was poorly differentiated or advanced ovarian cancer incidence trends in risk (OR = 3.12,95% CI = 0.97 ~ 10.61; OR = 2.21,95% CI = 0.95 ~ 5.12). 2.2 MMP-13 -77 A / G SNP of A / A, A / G, G / G genotype frequencies in ovarian cancer group and control group were 25.0%, 51.6%, 23.4% and 20.7%, 50.2 %, 29.2%, respectively, compared with no significant difference (P = 0.21); A, G allele frequency in ovarian cancer group and the control group no significant difference (P = 0.09). With the G / G genotype, A / A genotype was associated with epithelial ovarian cancer risk trends (OR = 1.51,95% CI = 0.94 ~ 2.41); while the A / G genotype does not alter epithelial the risk of ovarian cancer. Stratified according to histological type, and G / G genotype, A / A genotype significantly increased the incidence of mucinous ovarian cancer risk (OR = 9.88, 95% CI = 2.18 ~ 44.91), and an increase in slurry the risk of ovarian cancer incidence trends (OR = 1.86,95% CI = 0.95 ~ 3.64); stratified according to clinical stage, and G / G genotype, A / G and A / A genotype could significantly increased early epithelial ovarian cancer risk (OR = 1.95,95% CI = 1.02 ~ 3.73; OR = 2.62,95% CI = 1.28 ~ 5.39). 2.3 2LD program analysis showed that MMP-12 -82 A / G and MMP-13 -77 A / G SNPs in linkage disequilibrium (D '= 0.51). EH application software MMP-12 -82 A / G and MMP-13 -77 A / G SNPs haplotype analysis showed: MMP-12A/MMP-13G (53.3%), MMP-12A/MMP-13A (45.0%), MMP-12G/MMP-13G (0.9%) and MMP-12G/MMP-13A (0.8%) four kinds of haplotypes in epithelial ovarian cancer and control groups was no significant difference (P gt; 0.05). Conclusions: 1 VEGF-2578C / A and VEGF 936C / T gene polymorphism may be associated with China Northern women with epithelial ovarian cancer genetic susceptibility. 2 and the A / A genotype, MMP-12-82A / G genotype can significantly increase women in northern China epithelial ovarian cancer risk, and with the pathological type of ovarian cancer and age were significantly correlated, and the possible Epithelial ovarian cancer is a potential risk factor. 3 and G / G genotype, MMP-13-77A / A genotype increased in northern China women with epithelial ovarian cancer risk trends, and with the pathological type of ovarian cancer and clinical stage were significantly associated with their may increase the incidence of epithelial ovarian cancer risk. Four women in northern China MMP-12-82A / G and MMP-13-77A / G polymorphism in linkage disequilibrium. MMP-12A/MMP-13G is the most common haplotype, compared with MMP-12A/MMP-13G, MMP-12 A/MMP-13-77A haplotype with increased risk of EOC trend.
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