Dissertation > Excellent graduate degree dissertation topics show

Experimental Studies on Therapy of Lung Cancer by VEGF-C siRNA Mediated by Liposomes

Author: ZhangZuo
Tutor: QianYongYue
School: Suzhou University
Course: Department of Cardiothoracic Surgery
Keywords: Vascular endothelial growth factor C (VEGF-C) RNA interference Lung cancer Gene therapy
CLC: R734.2
Type: Master's thesis
Year: 2011
Downloads: 15
Quote: 0
Read: Download Dissertation


Objective: Lung cancer is the high incidence of respiratory malignancies, usually vivo gene mutation leads to changes in cell cycle and apoptosis inhibition. In recent years, many experimental studies have shown that VEGF-C plays an important role in tumor development. In this study, through in vitro synthesis of small molecules targeting VEGF-C mRNA interference RNA (short interfering RNA, siRNA) liposome-mediated transfection of human lung cancer A-549 cell line, observed VEGF-C mRNA and VEGF-C protein expression and cell growth and apoptosis. Method: the design and synthesis of VEGF-C gene sequence specific small interfering RNA by liposome-mediated transfection of lung cancer A-549 cells to silence the expression of VEGF-C. Transfection efficiency was observed with a fluorescence microscope and inverted microscope and morphological changes after transfection; siRNA and liposome detection experiment concentration of pure observation targeting VEGF-A-549 cells inhibited by MTT assay method, C siRNA via liposome conductor infected lung adenocarcinoma A-549 cell growth inhibition and cell growth curve. Using semi-quantitative reverse transcriptase polymerase chain reaction (reverse transcriptase polymerase chain reaction, RT-PCR) and protein immunoblot method (by western blotting) Determination of VEGF-C mRNA and protein expression. VEGF-C siRNA A-549 cell apoptosis was detected by flow cytometry. Transmission electron microscopy to observe changes in cell ultrastructure of exogenous VEGF-C siRNA transfected A-549 cells. Results: liposome-mediated siRNA transfected lung adenocarcinoma A-549 cells. Experimental concentrations of pure siRNA and liposomes of A-549 cells without significant toxicity. Liposome-mediated VEGF-C siRNA transfected lung cancer cells after 48h, the experimental group cells adherent infiltration decline in their ability to grow, some cells off shrinkage, round cell morphology, reduced in size, refraction enhanced blank control group and the experimental control group of normal cell growth. VEGF-C gene sequence specific siRNA can effectively inhibit VEGF-C mRNA and protein expression in lung adenocarcinoma A-549 cells, and the differences were statistically significant (P lt; 0.05) compared with the control group and the experimental control group. Down the expression of VEGF-C in vitro significantly inhibit the growth and proliferation of A-549 cells in a concentration-dependent inhibition rate within a certain range of concentrations, lowered VEGF-C A-549 can induce apoptosis increase 48h after transfection, the experimental group growth inhibition rate (38.87%), the rate of apoptosis (19.31 ± 2.21)% was significantly higher than that in the control group (P lt; 0.05), typical of apoptotic cells after transfection with siRNA and found under a transmission electron microscope, the nuclear chromatin PACK, uneven distribution. Conclusion: RNA interference technology can effectively inhibit VEGF-C mRNA and protein expression of VEGF-C and lower lung adenocarcinoma A-549 cell proliferation, induction of apoptosis increased, providing theoretical support for the targeting of VEGF-C siRNA treatment of lung cancer, RNA interference as a treatment for lung cancer means worthy of further research and promotion.

Related Dissertations

  1. Polyethylenimine Conjugated Stearic Acid-g-chitosan Oligosaccharide Micelles for Antitumor Gene Therapy,R450
  2. The Clinical Research for the Effect of Iodine-125 Seeds Implanted in Advanced Bronchial Lung Cancer,R734.2
  3. Expression Dynamics of Pheromone Binding Proteins and Expression Influences by Mating and Knockdown of Cryl in Spodoptera Exigua,S433.4
  4. Molecular Cloning, Mrna Expression and Rnai of Nadph-Cytochrome P450 Reductase Gene in Helicoverpa Armigera (Hǘbner),S435.622.3
  5. Effects of BMPR-IB Gene Silencing by Small Interfering RNA on Apoptosis of Porcine Pollicular Granulosa Cells and Ecpression of BMP Pathy-Way-Ralted Genes,S828
  6. Inhibition of Myostatin (MSTN) Expresstion in Primary Porcine Fetal Fibroblasts by Lentivector-mediated RNAi,S828
  7. The Construction and Evaluation of a Novel Non-viral Gene Transfection System and Its Application in Mesenchymal Stem Cells Gene Recombination,R346
  8. Effects of 5-Aza-2-deoxycytidine on DNA Methylation and Expression of p16 and MGMT Gene in Lung Cancer Cell Line SPC-A-1,R734.2
  9. Methylation of p16 Gene in Plasma and Tissues from Non-small Cell Lung Cancer Patients, Demethylation and the Biological Behavior of Lung Cancer Cell and Transcription of p16 Gene,R734.2
  10. A Preliminary Study on the Regulation of Hepatoma Cell Biological Behavior by MCFP,R735.7
  11. The Clinical Value of Detection of Serum TPS, CEA, CYFRA21-1, NSE for Diagnosis in Patients with Lung Cancer,R734.2
  12. Study on the Expression and Correlation of Metallothionein and Matrix Metalloproteinase-2 in Non-small Cell Lung Cancer,R734.2
  13. The DNA Cloning and RNA Interference of Some Functional Genes in Laodelphax Striatellus (Fallen),S435.112.3
  14. The Effect of RNA Interference-mediated ERCC1 Gene on the Chemo-treatment Sensitivity of NSCLC in Vitro,R734.2
  15. Non-small Cell Lung Cancer Analysis of 133 Cases of CVATS Lobectomy,R734.2
  16. Clinical Observation of Pemetrexed for Treating Advanced Non-small Cell Lung Cancer,R734.2
  17. Combined Detection of Tumor Markers in the Diagnosis and Treatment of Lung Cancer to Determine the Clinical Significance,R734.2
  18. Exploreing the Mechanism of Xieshifeiaifang Ii on Treating Lung Cancer with Pleura Effusion,R734.2
  19. The Eukaryotic Expression Vector Construction of pIRES2-EGFP-hIL-12-HSP70 and Its Influence on the Growth of 7402 Liver Cancer Cell,R346
  20. The Study of Inhibtion Effect by shRNA Expression Vector on Herpes Simplex Virus Type 2 UL54,R346
  21. Improvement of the Result of Autologous Fat Transplantation in Facial Recontouring Surgery by Using "Cocktail" Therapy,R622

CLC: > Medicine, health > Oncology > Respiratory system tumors > Lung tumors
© 2012 www.DissertationTopic.Net  Mobile