Dissertation > Excellent graduate degree dissertation topics show

A Preliminary Study on the Regulation of Hepatoma Cell Biological Behavior by MCFP

Author: ZuoZuo
Tutor: WangSiYing;YangXiaoMing
School: Anhui Medical University,
Course: Pathology and Pathophysiology
Keywords: lentiviral vector MCFP RNA interference Human hepatocellular liver
CLC: R735.7
Type: Master's thesis
Year: 2011
Downloads: 4
Quote: 0
Read: Download Dissertation


In eukaryotic cell mitochondria is the main site of energy metabolism. Mitochondrial function depends on a variety of soluble substances across the mitochondrial inner membrane transport of the endometrium, which is mainly completed by the transfer of solute carrier (SLC). SLC located in the inner mitochondrial membrane to form mitochondrial transporter family, including SLC25 family. The SLC25 family members had found a total of 14 species, of which SLC25A40, also known as MCFP (mitochondrial carrier functional protein), is a new member which identified in the central nervous system. MCFP as a newly discovered gene, the research reported rarely home and abroad.In our research we found that the expression of MCFP in most liver cancer cell lines was highter than normail liver cells. In our pervious research the effective sequence of siRNA targeting MCFP gene was confirmed, the complementary DNA containing both sense and antisense Oligo DNA of targeting sequence was designed, synthesized and cloned into the pSiCoR vector. The resulting lentiviral vector containing MCFP shRNA was named pSiCoR–MCFP. The MCFP cell lines were transfected by pSiCoR -MCFP recombinant lentivirus and the stable transfected liver cancer cell lines and normal liver cell line were established. The interference effect of MCFP was analyzed by PCR and Western blot. They both confirmed that the expression of MCFP decreased in the stable transfected HepG2 cell line (HepG2-SiMCFP VS HepG2-Control, P <0.001). Using the stable HepG2 cell lines we detected changes of the cell behavior and the mitochondrial function. Conclusion that knockdown MCFP in HepG2 cells can suppress cell proliferation, hold back cell course at S, and accelerate cell apoptosis. Also the production of ATP reduced and the complex activity decreased significantly. In our research we detected changes of the cell behavior and the mitochondrial function in the stable transfect HepG2 cell line. It can be used for further study of the role of the MCFP gene in the regulation of HepG2 cell. In conclusion, we investigated biological function of MCFP, opening up new avenues of inquiry into mitochondrial protein functions and mitochondrial disease mechanism.

Related Dissertations

  1. Expression Dynamics of Pheromone Binding Proteins and Expression Influences by Mating and Knockdown of Cryl in Spodoptera Exigua,S433.4
  2. Molecular Cloning, Mrna Expression and Rnai of Nadph-Cytochrome P450 Reductase Gene in Helicoverpa Armigera (Hǘbner),S435.622.3
  3. Effects of BMPR-IB Gene Silencing by Small Interfering RNA on Apoptosis of Porcine Pollicular Granulosa Cells and Ecpression of BMP Pathy-Way-Ralted Genes,S828
  4. Inhibition of Myostatin (MSTN) Expresstion in Primary Porcine Fetal Fibroblasts by Lentivector-mediated RNAi,S828
  5. The DNA Cloning and RNA Interference of Some Functional Genes in Laodelphax Striatellus (Fallen),S435.112.3
  6. The Effect of RNA Interference-mediated ERCC1 Gene on the Chemo-treatment Sensitivity of NSCLC in Vitro,R734.2
  7. The Study of Inhibtion Effect by shRNA Expression Vector on Herpes Simplex Virus Type 2 UL54,R346
  8. The Influence of RNA Interference Suppression EphB4 Expression for the PANC-1 Cells of Pancreatic Cancer,R735.9
  9. Experimental Studies on Therapy of Lung Cancer by VEGF-C siRNA Mediated by Liposomes,R734.2
  10. The Functions of Chitinase Genes from Locusta Migratoria,S433.2
  11. Construction of Silence Vector for Cytochrome b Gene in Nucleus of Arabidopsis Thaliana and the Transformation,Q943
  12. Construction of RNA Interference Vectors of Pin Genes Determining Grain Hardness and Genetic Transformation in Wheat,S512.1
  13. Expression of the POU Domain Transcription Factor Oct4 in Human Transitional Carcinoma Tissue of Bladder and Its Effects on the Biological Behavior of Human Bladder Cancer Cell Line EJ,R737.14
  14. Lisianthus ACC synthase gene RNA interference,S682.19
  15. Silencing of SOCS1 Enhances Dc-mediated Anti-laryngocarcinoma Immunity,R739.65
  16. Research of Damage Efficiency by Livin/Survivin RNA Interference on Colorectal Cancer Cell Mediated by mPEGylated-chitosan Nanoparticles,R735.34
  17. Construction and Identification of MicroRNA Eukaryotic Expression Vectors Targeting Vsacular Endothelial Growth Factor,R735.7
  18. Study on the Function of FTH1 in the Course of Brucella Ovis 019 Infecting RAW264.7 Macrophages,S852.61
  19. Study of Transgenic Technology in Processing Tomato Resist Cucumber Mosaic Virus,Q943.2
  20. Cloning and Construction of RNA Interfere Vector of Ice Recrystallization Inhibition Gene Form Lolium Perenne,S543.6

CLC: > Medicine, health > Oncology > Gastrointestinal Cancer > Liver tumors
© 2012 www.DissertationTopic.Net  Mobile