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Effect of Different Levels of Zinc Deficiency on the Development of Heart in Rat Fetus and the Possible Mechanism

Author: KangFenHong
Tutor: HeXiaoYu
School: Fujian Medical
Course: Obstetrics and Gynaecology
Keywords: Zinc deficiency Heart malformations ALP Serum zinc concentration Placenta Metallothionein Zinc transporter protein
CLC: R714.5
Type: Master's thesis
Year: 2009
Downloads: 59
Quote: 0
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Abstract


Zinc is an essential trace element purpose, one of the mother during pregnancy zinc homeostasis in normal pregnancy and fetal growth and development is extremely important, zinc deficiency can lead to body dysfunction, behavioral abnormalities and birth defects. Birth defects and congenital heart disease is the most common diseases, the present study was to construct different levels of zinc pregnant rat model to explore the heart of zinc deficiency on embryonic developmental toxicity in rats and its role in the threshold dose and dose - effect relationship, and to explore its possible mechanism of action, is designed to correct zinc supplement and clinical monitoring and prevention of cardiac malformations provide experimental basis. Methods healthy female SD rats of clean grade 50, were randomly divided into five groups: a low zinc group, two low-zinc group, 3 medium low zinc group, four edge low zinc group, five normal zinc group fed with zinc different feed. After 25 days of feeding zinc depletion mating to day 19 of gestation Caesarean Section mouse embryo heart, while specimens from pregnant rats and placental blood samples. Pathological observation of fetal cardiac malformations situation; atomic absorption spectrometry pregnant rat serum zinc values; alkaline phosphatase detection kit pregnant rat serum AKP activity; RT-PCR assay placenta MT1 and ZNT1 mRNA relative expression. Results 1. Pregnant rats each low zinc serum zinc values ??and serum AKP activity and normal zinc group were significantly different (both P lt; 0.05), with zinc content of the feed increases, and the increase in serum AKP activity more is obvious. Two in each group were found in mouse embryos absorbed fetus, birth defects and fetal death, over medium low zinc group compared with the normal zinc was significantly increased (both P lt; 0.01). 3 above average low zinc group compared with the normal zinc, fetal heart malformations were significantly increased (both P lt; 0.05); the low zinc group, there was significant difference (both P lt; 0.05). 4 above average low zinc group placenta MT1 and ZNT1 mRNA abundance relative expression more frequently zinc group were significantly lower (both P lt; 0.05). 5 mouse embryonic cardiac abnormalities intraday MT1 and ZNT1 mRNA abundance relative expression was significantly lower than normal embryos (both P lt; 0.01). Conclusions 1. Zinc deficiency in rats after feeding formula feed general performance and the value of serum zinc and serum AKP was significantly decreased, indicating that zinc deficiency rat model of success. (2) the same low zinc case, serum AKP activity than serum zinc values ??changed significantly, indicating that the AKP activity and blood serum zinc values ??combine to more accurately reflect the level of zinc in the body. 3 above average poor low zinc Embryology mouse embryos and increased incidence of cardiac malformations, and with low zinc levels increased aggravation. Tip pregnant mice low zinc have teratogenic effects on the embryo, and the obvious presence of toxic effects of dose - response relationship for the prevention and treatment of zinc deficiency above average should pay attention to. 4 above average low zinc Embryology mouse placenta MT1 mRNA expression was decreased, suggesting that low zinc may reduce the body's zinc reserves and through antioxidant capacity, affecting the normal development of embryonic heart. 5 above average low zinc Embryology mouse placenta ZNT1 mRNA expression was decreased, suggesting that low maternal placental zinc transporter of zinc can lower the ability of mouse embryos to reduce the blood supply of zinc, increased high oxidative stress, increased cardiac malformation rate of embryo . 6 embryonic rat cardiac malformations compared with normal mouse embryo and placenta ZNT1 mRNA expression of MT1 decreased, suggesting that low zinc only affect maternal placenta MT1 and ZNT1 related proteins such as zinc may result only when embryonic rat cardiac malformation.

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CLC: > Medicine, health > Obstetrics and Gynaecology > Obstetrics > Fetus
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