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Objective: to establish dog model of traumatic brain injury, the use of microdialysis technique in the injured brain different times of the day and the sampling and analysis. To dynamic changes in the metabolism of certain substances in the extracellular fluid index content. Such as glucose, lactate, glycerol, etc.. Metabolism of these substances from the understanding of the brain damage indicators such as changes in the law and its clinical significance, and explore the microdialysis technique in local biochemicals monitoring of brain injury. Methods: 6 adult healthy dogs, anesthesia to surgical fenestration exposed dura mater. The freefall the epidural impact method to making mild and severe brain injury model, application microdialysis technique dynamic constant speed collection of brain damage local ECF collected once in each interval of 30min, three specimens were collected before injury. 12 specimens collected 6h after injury. Contralateral normal brain tissue at the same time as a reference, will collect the dialysate Goryeong analysis, so as to observe the of craniocerebral trauma state where glucose (Glu), lactate (Lac), pyruvate (DYR), glycerin (Gly) contents and dynamic changes of substance. Results: continuous detection by microdialysis method in the extracellular fluid Glu, Gly and Lac concentration, brain damage observed after Glu content decreased compared to the contralateral reference decreased significantly 90min after injury, reached the peak, followed by gradual recovery . Lac content after injury Glu opposite change, but the change is slower. The brain ECF Glu and Lac content changes related to the degree of injury, damage more severe the most obvious change, the longer the duration can be as important basis to determine the extent of the injury and brain edema. Also prompted more obvious changes in the ratio of Glu and Lac (UG), to better reflect the brain tissue redox state and ischemic level. Glycerol did not change significantly, the light injury severity. Conclusions: 1. Microdialysis technique is a minimally invasive, security, new means of monitoring, continuous monitoring of metabolic brain damage local biochemicals content. 2 after brain injury the ECF in glucose content lower lactic acid levels increased, both were contrary to the dynamic changes, and speculated that the reason may lead to brain damage brain tissue glycolysis significantly enhanced. 3. ECF glucose and lactic acid content in the monitoring of brain injury, as the judgment of the injury and the prognosis of important biochemical indicators. 4.Glu Lac ratio change is more obvious, and can be used as a more sensitive indicator of potential.
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