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Objective: To establish due to rabbit intestinal perforation peritonitis model to study intestinal perforation due to peritonitis caused by the major organs of the body liver, lung, kidney damage, the related treatment measures and to explore its therapeutic effect. Methods: the first part of the test of 20 healthy rabbits were randomly divided into 2 groups of sham-operated group (A), intestinal perforation group (B), through the establishment of a control group, to establish the model of peritonitis caused by intestinal perforation, arterial blood gases, blood biochemical, pathological and TUNEL studied by means of peritonitis for the major organs of the rabbit liver, lung and kidney damage. The second part of the test of 50 healthy rabbits were randomly divided into five groups of 10: the intestinal perforation repair group (C), imipenem treatment group (D), ulinastatin treatment group (E), the Xuebijing treatment group (F) and imipenem joint Ulinastatin he small, blood will net treatment group (G group), repair the perforation and the use of imipenem, ulinastatin, blood will net and other drugs The treatment of perforation peritonitis to study the protective effect. Results: the first part test. Arterial blood gas results: the sham group (A) with perforation group (B) Comparison of blood pO2, blood pCO2 differences (P <0.05); blood lactate Lac sham-operated group (A) comparison with perforation group (B group) the existence of significant differences (P <0.01); remaining indicators no difference (P> 0.05). 2. The blood biochemical detect: the sham group (A) and perforation group (B) Comparison of blood AST differences (P <0.05), ALT, BUN there are significant differences (P <0.01) .3 liver HE staining: Group A (sham group): mainly hepatic sinusoid congestion, some liver cells with mild edema, no significant necrosis, no significant inflammatory cell infiltration in the portal area and lobular. Group B (perforation): mainly to the remarkable expansion of hepatic sinusoid and central venous congestion, liver the cells widely height edema, visible focal necrosis and bridging necrosis, portal area and lobular significant inflammatory cell infiltration, liver vacuolar deformation, visible hepatocyte nuclear fragmentation, the lobule structure is still intact, the hepatic cord disarranged. The kidney HE staining: A group (sham group): glomeruli and tubules no obvious abnormal B group (perforation group of): tubular mild edema, part of the the perforation group of patients visible tubular severe edema, glomerular no obvious lesions. Liver TUNEL assay: the sham group (A) TUNEL assay results: liver tissue two dark blue points can be regarded as a physiological apoptosis at low field, is the apoptotic cells, high power field fresh See. Perforation group (B) TUNEL assay results: liver tissue the multiple scattered blue-stained apoptotic cells in high power field, A group of apoptotic cells increased significantly low vision is more obvious. The second part of the test. Arterial blood gas results: perforation repair group compared with the perforation group differences (P <0.05) in blood lactate determination on the other there is no difference (P> 0.05). Imipenem treatment group (D) with perforation group comparison, there are differences in the partial pressure of oxygen, carbon dioxide partial pressure (P <0.05), and the other was no difference (P> 0.05). Ulinastatin treatment group (E), the blood will be the net treatment group (F) with perforation group comparison, blood lactate determination significantly different (P <0.01), the other was no difference (P> 0.05 ). Imipenem combined with ulinastatin and the Xuebijing treatment group (G group) and perforation group comparison, the partial pressure of oxygen on the differences (P <0.05), blood lactate determination on the existence of significant difference (P < 0.01), and each of the other there was no difference (P> 0.05). Blood biochemical detection: perforation repair group (C group) compared group and perforation group (B), AST, ALT, BUN determination of differences (P <0.05). Imipenem treatment group (D), ulinastatin treatment group (E), the blood will be the net treatment group (F) and perforation group (B) compared differences in AST, ALT, determination Xuebijing treatment group (F) compared perforated in BUN differences (P <0.05) (P <0.05); Compared to imipenem combined with ulinastatin and blood will net treatment group (G group) with perforation group (B), in the determination of AST, ALT, BUN there are significant differences (P <0.01). 3. Liver HE staining: CG group (treatment group): congestive visible hepatic sinusoid varying degrees of liver cells with mild to moderate edema, liver cytoplasmic loose, occasionally vacuolar degeneration visible point, focal necrosis, no bridging necrosis. Kidney HE staining: tubular mild edema in all treatment groups, some cases of perforation group visible tubular severe edema, glomerular no significant lesions, perforation group and the treatment group was no significant difference between the lesion. 4. Hepatic TUNEL detect: liver tissue for each treatment group scattered moderate amounts of deep blue points in the low-field, high power field in the number of apoptotic cells was significantly less than the B group. Renal TUNEL assay: wild-low renal tissue scattered apoptotic cells in the renal tubular epithelium. High power field, the same can be seen in the B group and the treatment group (CG group) there was no significant difference, and the lesions were mild apoptotic cells and liver tissue significantly reduced. Conclusion: The test successfully established intestinal perforation-induced peritonitis model in this model on the basis of the correlation detection found resulted in varying degrees of damage of the liver, lung, kidney, intestinal perforation induced peritonitis. Repair intestinal perforation and the use of imipenem ulinastatin, blood will net a certain extent, and other drugs can reduce organ damage.
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