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[Objective] To observe the protective effect of the additional Danshen injection (SM) and 1,6 - diphosphate (FDP) St.Thomas liquid continuous low temperature trace perfusion of the isolated rat heart. Try looking from a save during coronary effluent predict heart preservation quality biomarkers (biomarker). [Methods] SD rats were randomly divided into 4 groups: control group, SM group, FDP group, SM the FDP group. The isolated rat heart after cardioplegia with cold St.Thomas liquid, non-circulating Langendorff perfusion 30min, then according to the packet St.Thomas liquid, the St.Thomas liquid SM St.Thomas liquid FDP, St.Thomas liquid SM FDP cardioplegic cardiac, and 4 ° C under the conditions of continuous perfusion (20μl/min) save after 6h again perfusion for 30 min before and after cardiac function index was measured in vitro heart preservation: the maximum rate of change of left ventricular pressure (± dp / dt max ), left ventricular developed pressure (LVDP), heart rate (HR) and coronary flow (CF), and the preservation during coronary effluent lactate dehydrogenase (LDH), creatine kinase (CK) activity and heart tissue moisture content. Remaining protein in the coronary effluent DOC-TCA precipitation concentrated SDS-PAGE and silver staining, looking for differences in protein can predict heart preservation quality measured differences in protein molecular weight, and application the ImageJ software processing protein gel silver stained image gray value analysis of differentially expressed proteins zone with all indices correlation. [Results] low temperature continuous perfusion preservation in the complex irrigation period of 20 min, SM Group, FDP group SM the FDP group LVDP -dp/dt max values ??were higher than the control group (P <0.05), the SM group, FDP group The dp / dt max higher than those in the control group (P <0.05), the SM group, FDP group, SM the FDP group dp / dt max recovery rate higher than that of the control group (P <0.05). HR and CF values ??are no significant differences (P> 0.05) in the four groups between. SM group, FDP group, of, SM FDP group LDH enzyme activity were lower than the control group (P <0.05), the FDP group, of, SM FDP group CK enzyme activity than the control group (P <0.05). Heart water content value of the SM Group, FDP group, the, SM FDP group were lower than the control group, but there was no significant difference (P> 0.05). By analysis of the silver stained SDS-PAGE results, to find the difference protein zones: A (15.1kDa), B (16.6kDa), C (30.2kDa) and D (39.8kDa), wherein the zone A, B and C CK enzyme activity was positively correlated. [Conclusion] The application of low-temperature continuous microinfusion preservation methods, add SM St.Thomas solution or the FDP can improve heart preservation effect, both Wu with effect with no significant difference. Preliminary analysis to save during coronary effluent differences protein District with A, B and C, and the donor heart myocardial damage related needs further study.
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