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Objective: 1. Single segment part destruction posterior lumbar stabilization structure manufacturing early New Zealand rabbit lumbar disc degeneration model for pathological examination of rabbit lumbar intervertebral disc and disc magnetic resonance imaging (magneticresonance imaging, MRI) observed, comparing different time points differences between early degenerative lumbar disc pathology, imaging change; intradiscal injection of adenovirus (adenovirus, Ad)-mediated human insulin-like growth factor 1 (human insulin-like growth factor-1 hIGF-1) gene, cytokine hIGF-1 in the treatment of early lumbar disc degeneration the comparative observation the Ad-hIGF1 gene, hIGF-1 factor for MRI and pathological changes after early lumbar disc treatment for lumbar degenerative diseases, early prevention and treatment provide experimental basis. Methods: 1. Choose the health of the 6-month-old, 60 New Zealand white rabbits were randomly divided into the experimental group (30) and the control group (30), the experimental group carried out a surgical operation, the single segmental destruction spine, interspinous dead muscle, ligaments and intervertebral disc control group only skin incision suture, two groups of animals under the same conditions of rearing, respectively after 2 months, 4 months, 6 months for the experimental group and the control group BOC magnetic resonance imaging to detect and drawn for pathological examination, MRI, pathological changes their comparison; 2. choice of 6-month-old healthy 32 New Zealand white rabbits, its operation caused by degenerative lumbar early surgery, were randomly divided to turn gene group (16 Zhi) and growth factor group 1 (8 Zhi), control group (8 rats), all animals under the same conditions after keeping four months in the GM group disc injection of Ad-hIGF1, growth factor group intervertebral disc within injection hIGF1, control group injected with saline. Two weeks after injection, respectively, 1,2 derived observe hIGF1 expression, and in February after injection of contrast MRI and pathological examination results in groups. Results: 1.MRI 2 months of the experimental group and the control group no significant difference 4 months of the experimental group, experimental group six months of lumbar disc surgery segment T2WI showed low density signal change over 4 months, 6 months Obviously, some were black intervertebral disc change, all experimental animals lumbar spine did not appear scoliosis, kyphosis and disc space narrowing. Hematoxylin - eosin staining (Hematoxylin and Eosin Staining HE), the experimental and control groups compare two cartilage endplate matrix changes, endplate April the cell arrangement owe rule, the endplate cells significantly reduced the number of layers, endplate thinner cell degeneration appears endplate vascular area decreased 6 months endplate cell layers to further reduce or even disappear while the nucleus pulposus cell degeneration, reduce the number and boundaries unclear. Periodic acid-Schiff reaction (Periddic Acia-Schiff reaction, PAS) staining, measured gray value, the control group cartilage endplate lowest gray value, experimental group two months, followed by 4 months of further high, 6 months when the highest gray scale value. Transgenic group disc two weeks after the injection, Jan. harvested after February found that the the intervertebral disc hIGF1 significantly expression detected by RT-PCR, the strongest of which two weeks in January, February, followed by gradually growth factor group two weeks after the detection no hIGF1 expression. 3 transgenic group, the growth factor group, the control group surgery lumbar nucleus pulposus T2WI showed low density signal change, which transgenic group compared with the group of cytokines, the signal value of the control group, the growth factor group, no significant difference between the control group . HE staining cytokines group, between the control group and the morphological structure of the disc without distinction. Compared with the transgenic reduced nucleus pulposus cells, unclear boundaries, the number of end-plate cells, no significant differences in morphological changes. The PAS special dyeing prompted transgenic group, growth factor group, the control group between the cartilage endplate staining difference is not obvious. Conclusion: 1. Destruction can induce ligament, interspinous ligament, and vertebral disc muscle ended on the rear side of the spine of the lumbar spine lumbar cartilage endplate, nucleus, annulus fibrosus slowly progressive degeneration. 2 gene therapy with Ad-hIGF1 the rabbit lumbar early disc degeneration has improved to some extent.
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