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Objective: To study breast cancer tissue of Pokemon mRNA, sequence-specific transcription factor c-myc expression, and to explore the relationship between clinicopathological factors in its expression and breast cancer, the correlation between the analysis of of Pokemon mRNA with c-myc expression. Methods: In situ hybridization (ISH) detected 45 cases of breast cancer, 20 paracancerous breast tissue of Pokemon mRNA expression in 20 cases of normal breast tissue, analysis of the relationship of its expression with clinicopathological parameters; Using immunohistochemistry staining (SP method) detected 45 cases of breast cancer, in 20 paracancerous breast tissue, c-myc expression in 20 cases of normal breast tissue, analysis of the relationship of its expression with clinicopathological parameters; analysis Pokemon mRNA and c-myc Correlation of expression. Results: 1. Pokemon mRNA in breast cancer cells mainly expressed in the cytoplasm. Pokemon mRNA expression in breast cancer tissue positive rate was 71.11% (32/45), significantly higher than in adjacent breast tissue in 30% (6/20), normal breast tissue 20.0% (4/20) (P <0.05); breast Pokemon mRNA expression and axillary lymph node metastasis, histological grade (P <0.05), Pokemon mRNA positive expression rate (100.0%, 9/9) in the level III breast tissue than in grade Ⅱ breast cancer tissue positive expression rate (71.43%, 15/21) and grade Ⅰ breast cancer tissue positive expression rate (53.33%, 8/15), there was significant difference between the three groups (P = 0.0253). With patient age, tumor size, TNM stage, no significant correlation (P> 0.05). 2.c-myc expression in the cytoplasm of breast cancer cells. Positive rate was 86.67% (39/45), c-myc expression in breast cancer tissue was significantly higher than in adjacent normal tissues, 60% (12/20), normal breast tissue 50% (10/20) (P <0.05 ). C-myc expression was no significant difference (p> 0.05) between adjacent noncancerous breast tissue and normal breast tissue. c-myc expression and breast cancer axillary lymph node metastasis, tumor size was significantly related (P <0.05), and c-myc in cancer> 2cm positive expression rate in breast cancer tissue (12.5%, 5/40) less than its cancer ≤ 2cm positive expression rate (50.0%, 15/30), the difference between the two groups was significant (p = 0.002). With the patient's age, histological grade, TNM stage was no significant correlation (P> 0.05). 3.45 cases of breast cancer cytoplasmic Pokemon mRNA and c-myc expression was positively correlated (γ = 0.585, P <0.05). Conclusion: 1.Pokemon mRNA protein upregulated in breast ductal carcinoma; overexpression with axillary lymph node metastasis, histological grade (P <0.05), of pokemon mRNA in protein upregulation in breast cancer. Its upregulation may be related to the incidence of breast cancer and invasion metastasis. 2.c-myc overexpression in breast ductal carcinoma; overexpression ductal carcinoma tumor size and lymph node metastasis, and the prompts to c-myc overexpression is closely related to the development of breast ductal carcinoma. 3 Pokemon mRNA in ductal breast cancer, c-myc expression was positively correlated. pokemon may be an important regulatory factor c-myc. That they may participate in the occurrence of breast cancer development process. 4.Pokemon mRNA, c-myc indicators to assess the clinical and pathological features of ductal breast cancer, both combined detection of help from the many ways to understand the degree of malignancy of ductal breast cancer, and helps determine the prognosis of patients.
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