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Background: Since the history of the human line medical records, neck and shoulder pain, low back pain is, as they relate to the contents of the disc degeneration is the most common cause of neck pain. 1934, Mixter and Barr [1] by first confirmed by surgery and cure of lumbar disc sciatica due to nerve root, thus creating a so-called \The classic surgical discectomy for more than 70 years to cure countless disc disease patients, however, more than 70 years, thousands of relevant literature published worldwide each year, each related orthopedic meetings disc disease is still the most important one of the topics. This fact also shows that there are still many problems in the treatment of degenerative disc disease, need further research and exploration. In recent years, the study of tissue engineering is also extended to the field of spine surgery, foreign scholars to carry out basic research work related to tissue engineered intervertebral disc. Although the number of published studies is still small, however, reaction welcome the prospect of this study. Objective: Research and intervertebral disc degeneration or regeneration of the relevant environmental factors as well as the biological behavior of the intervertebral disc tissue and cells, observed platelet-derived growth factor on cultured intervertebral disc cell proliferation and matrix synthesis, from the cellular level, molecular biology and genetic level degeneration of intervertebral disc gene therapy and tissue engineering research. Methods: 1, separation and digestion of rat intervertebral disc tissue single cells and complete primary cells were inoculated. Passaged in primary cell fusion to establish the control group and the experimental group (applied 1μg / L, 10μg / L and 100μg / L, 1000μg / L of different concentrations of platelet-derived growth factor stimulation of growth). 3 HE, toluidine blue staining statutory time cell morphology was observed under an inverted microscope, proliferation changes, immunocytochemistry staining of type II collagen expression. 4, using the absorbance values ??of MTT (thiazolyl blue colorimetric) method for the determination of disc cells and cell growth curve. 5, the passage cells digest as a single in S phase (DNA synthesis phase) cell content by flow cytometry. Statistical software analysis concluded. Results: 1 inverted phase contrast microscope continuous observation, 10 days more than 95% of the cells adherent primary cells for the spindle, extended pseudopodia; passaged by trypsin digestion, adherent cells growing faster adding platelet-derived growth factor group of disc cells convergence time, the acceleration of growth. 2, HE staining cells mostly fusiform, pseudopodia extended nucleus is round or oval around the nucleus and cytoplasm more vacuoles; toluidine blue staining nucleus is purple, the cytoplasm stained dark blue . Collagen type II Immunocytochemistry showed positive results. 3, MTT method determination of intervertebral disc cells absorbance (OD) values ??after analysis of variance test shows that, F = 20.276, p = 0.000, the difference between the five groups was significant. Pairwise comparisons drawn between five groups was no significant difference between the control group, 1μg / L group, and 10 μg / L group, between the group 1000μg / L and 100μg / L group was no difference in the control group, 1μg / L group , 10μg / L group 1000μg / L group, 100μg / L group, the difference was statistically significant, statistically significant difference (P lt; 0.05). With the increase of the concentration of growth factors, the positive correlation in the number of cells in the S phase by flow cytometry. rR-PDGF-AA can improve the proliferative activity of the cells, and a dose-effect relationship in the effective concentration range. Conclusion: In this study, the culture of disc cells provide a simple and effective method, platelet-derived growth factor on the proliferation of disc cells cultured in vitro, a matrix of collagen and proteoglycan synthesis has a role in promoting, can significantly improve the activity of mitochondria, increase in the percentage of cells in S phase, and its effect was positively correlated with the dose and the time within a certain range.
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