Dissertation > Excellent graduate degree dissertation topics show

Study on the Gene Therapy for Severe Traumatic Brain Injury Through A20

Author: WuXiaoHua
Tutor: YangXiaoFeng
School: Zhejiang University
Course: Surgery
Keywords: Traumatic brain injury Apoptosis A20
CLC: R651.15
Type: Master's thesis
Year: 2008
Downloads: 24
Quote: 0
Read: Download Dissertation

Abstract


The purpose of the neuronal apoptosis after traumatic brain injury is one of the important ways of cell loss after injury , how to suppress apoptosis after injury so as to preserve more neurons to get better neurological outcomes have been research scientists one of the key points . A20 gene is a zinc finger protein gene family can antagonize TNFR -mediated death receptor-mediated apoptosis pathway . This study by the the A20 genes upregulated after liposomal transfection makes traumatic brain injury , so as to study the protective effect of A20 anti-apoptotic genes in traumatic brain injury brain . Method for the experimental group and the control group of 35 SD rats produced severe traumatic brain injury model after the experimental group and the control group of rats were injured after 30 minutes in stereotactic injection to multi- point damage the stoves surrounding cortex and damage foci liposome -pcDNA3.1-A20 and liposome - pcDNA3.1 empty plasmid . The two groups of animals at postoperative 12h, 24h , 48h , 72h , 168h 5 of each rat brain sliced ??immunohistochemistry assay A20 gene expression and injury apoptosis ; each group remaining 10h rats at 1, 2, 3, 4 weeks after injury , inclined plane test test of nerve function . Results (1) immunohistochemistry visible damage foci of experimental animals surrounding A20 expression after injury significantly higher than those in the control group ( P <0.01 ) . Apoptosis (2) after traumatic brain injury : traumatic brain injury seen after ipsilateral cortex , the distribution of the number of apoptotic cells in hippocampus , cortex most concentrated damage peri ; groups apoptosis at 72 hours after injury hours to reach the peak . Injury of experimental rats after 12 hours , 24 hours , 48 hours and 72 hours the number of neuronal apoptosis compared with the control group was significantly lower (P <0.01 or 0.05 ) . ( 3) 1, 2, and 3 weeks of the injury , the inclined plane test the critical angle of the experimental group and the control group of rats was not statistically significant (P> 0.05); injury four weeks , the experimental group Animal critical angle is greater than the control group ( P <0.05). Conclusions on traumatic brain injury in experimental animals liposome -mediated the A20 gene therapy can play a neuroprotective effect of anti- injury apoptosis .

Related Dissertations

  1. The Study on Cryopreservation and Mechanism of Freezing Injury on the Spermatozoa of Coelomactra Antiquate,S968.3
  2. The Anti-tumor Effect of CADPE and Inducing Apoptosis in Human Gastric Cancer Cells,R735.2
  3. Cloth and β- elemene combined administration of anti-tumor effect and mechanism of celecoxib,R96
  4. TRAIL in the regulation of tumor invasion CD4 ~ CD25 ~ Treg,R730.2
  5. The Study of Lead Exposure on Cyprinus Carpio Ovary Epithelial Cells,X174
  6. Effects of Diclazuril on G3PDH in Second-generation Merozoites of Eimeria Tenella,S858.31
  7. The Effects and Preliminary Study of PCV2 on Ca2+ Signal in Lymphocytes of Piglets,S858.28
  8. Effects of Angiogenic Factor on Follicular Angiogenesis and Development in Sexual Maturity Mice,S852.2
  9. Expression of β-Catenin in Pig’s Ovary and the Effect of β-Catenin on Porcine Granulosa Cells Apotosis and Steroidogenesis Related Enzyme,S828
  10. Induced Combination Apoptosis by FB1 and AFB1 in Vero Cell,S856.9
  11. The Apoptosis Mechanism Induced by Aflatoxin B1 and Deoxynivalenol in Primary Hepatocyte of Cyprinus Carpio,S856.9
  12. Effects of BMPR-IB Gene Silencing by Small Interfering RNA on Apoptosis of Porcine Pollicular Granulosa Cells and Ecpression of BMP Pathy-Way-Ralted Genes,S828
  13. The Effects of Don on Proliferation, Differentiation, and Apoptosis of Chondrocytes in Chicken,S858.31
  14. The Effect of Foxol on Apoptosis of Mouse Granule Cells,S865.13
  15. Study of Melittin on Tumor Inhibition and Part of Mechanism of Human Hepatocellular Carcinoma HepG-2 Xenograft in Nude Mice,R735.7
  16. The Protective Effect of Rat Myocardial Ischemia/reperfusion Injury Following Bone Marrow Mesenchymal Stem Cell Pretransplantation for 1 Week,R542.22
  17. Expression of WW Domain Containing Oxidoreductase Gene in Cholangiocarcinoma and Its Effect on the Biological Behavior of Cancer Cell Line QBC939,R735.8
  18. The Effects of (180F-FDG on the Apoptosis of Eca-109 Esophageal Cancer Cell Line,R735.1
  19. The Signal Mechanism Underlying DON Induced Apoptosis in BHK-21 Cells,S856.9
  20. The Expression and Mechanism of NF-κ B and IL-6 after 90% Portal Vein Ligation in Rats,R657.3
  21. The Study of Mitochondrial Signal Mechanism Induced Apoptosis by Don in Human Colon Cancer Cells,R735.35

CLC: > Medicine, health > Surgery > Of surgery > Head and Neurosurgery > Brain > Traumatic brain injury
© 2012 www.DissertationTopic.Net  Mobile