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Study on the Effect Hemoglobin Toxic Effect of Cerebral Hemorrhage in Rat with Qing Jie Yu Du Capsule

Author: WuXiaoLei
Tutor: FengXueGong
School: Beijing University of Traditional Chinese Medicine
Course: Chinese medical science
Keywords: Cerebral hemorrhage Hemoglobin Toxic effects Clear solution Ecchymosis capsules
CLC: R285.5
Type: Master's thesis
Year: 2009
Downloads: 47
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Abstract

Objective: To study the observation the clear solution Ecchymosis capsules hemoglobin toxicity after intracerebral hemorrhage understand detoxification treatment method of cerebral hemorrhage and non-detoxification treatment method differences clearly tongfu diuresis Qingreliangxue should cerebral blood stasis drug treatment reasonable method of prevention and treatment of cerebral hemorrhage, and thus improve the clinical efficacy and lay a good foundation for the clinical application of \Method: 1. Experimental animals and groups of male Wistar rats ,250-300g, were randomly divided into five groups (sham operation group, model control group, group of oral Naoxuekang, a clear solution Ecchymosis capsules high-dose group and the clear solution stasis Capsule in the low-dose group) n = 5. (2) Preparation of an animal model of cerebral hemorrhage group, the autologous fresh blood about 50 ul was injected into the right caudate nucleus; sham-operated group, 50 ul of the right caudate nucleus injected with saline. 3 test drug and dose design clear solution Ecchymosis capsules, prepared by the China Academy of Chinese Medical Sciences, 0.6g / capsule. The clear solution Ecchymosis capsules Clinical daily dose of 5.4g, adult weight by 60 kilograms, equivalent to 0.09g/kg; translated by animals and humans kg body weight dose equivalent, rat dose using 0.5g 0.25g/kg high the low dose group, intragastric volume of 10ml/kg, concentrations were 0.05,0.025 g / ml. Naoxuekang oral solution, Oasis Shenyang Pharmaceutical Co., Ltd., batch number: 20070503. Cerebral Oral Liquid, clinical daily dose of 30ml, the calculation of the adult body weight by 60 kg, equivalent to 0.5ml/kg; translated by animals and humans kg body weight dose equivalent rat dose 2.7ml/kg, intragastric volume of 10ml/kg administration take 2.7ml diluted to 10 ml. (4) the method of administration modeling animals in group 6 hours after administration of each drug group were given the corresponding concentrations of the drug, the model control group and sham operation group was given volume of distilled water (10ml/kg). Each group was given 1 times / day. Medication to postoperative 1d, 3d, 7d, 14d respectively. OUTCOME MEASURES brain water content observed the clear solution Ecchymosis capsules cerebral edema intervention; peroxidation product malondialdehyde (MDA) were measured by observing the animals, free radical scavenging enzymes - superoxide dismutase (SOD) content heme oxygenase H0-1 expression, in order to estimate the degree of oxidative damage; observation of changes in the animals neurological symptoms, evaluation clear solution Ecchymosis capsules protective effect on nerve tissue. Results: 1. Neurological symptoms rated sham-operated rats no obvious neurological deficit score was 0, the clear solution Ecchymosis capsule and Naoxuekang oral group 1d to 14d neurobehavioral scores low in the model control group. 2 heme oxygenase H0-1 expression in the model control group and the treatment group in the postoperative 1 to 14d hematoma by brain tissue around the heme oxygenase -1 (H0-1) positive cells compared with sham group , in which the model control group was significantly higher (P <0.05), oral Naoxuekang group postoperative 3d was significantly higher (P <0.05); compared with the model group, the rest of the group (except for the days when the brain Blood Oral Liquid group, 3d clear solution the Ecchymosis capsules low-dose group) perihematoma heme oxygenase -1 (H0-1)-positive cells was significantly reduced (P <0.05); between the group of drugs no significant difference was not statistically significant. Brain water content of cerebral hemorrhage in the the postoperative 1d to 14d brain tissue water content higher than the sham-operated group, reached the peak at 3d after which the model control group in 1D, 3d, 7d difference significant (P <0.05); each to drug group brain water content varying degrees lower than the model control group, one of the clear solution Ecchymosis high dose group in 3d after brain tissue water content with the model control group compared to the significant difference (P <0.0); was no significant difference between the group of drugs. Results of 4.SOD content model control group bleeding brain tissue SOD were significantly lower than in the sham operation group, bleeding brain tissue in rats with intracerebral hemorrhage around the anti-lipid peroxidation decreased. The clear solution of Ecchymosis capsules and Naoxuekang oral intracerebral hemorrhage in rats can significantly enhance the anti-lipid peroxidation medication group medication to postoperative 3d model control group (P <0.05) were significantly higher than in brain tissue SOD. 5.MDA content results show that the model control group bleeding brain tissue MDA was significantly higher in the sham operation group, cerebral hemorrhage induced by hemorrhagic brain tissue surrounding lipid peroxidation products increased significantly. The clear solution of Ecchymosis capsules and Naoxuekang oral intracerebral hemorrhage in rats anti-lipid peroxidation, can significantly enhance postoperative 1d 3D the, 7d day brain tissue content of MDA was significantly lower than the model control group (P <0.05) . Conclusion: The clear solution the Ecchymosis capsules can be significantly lower cerebral hemorrhage in brain water content; reduce bleeding brain tissue surrounding lipid peroxide (MDA), enhanced superoxide dismutase (SOD), anti-lipid peroxidation to reduce the number of bleeding brain tissue heme oxygenase H0-1 positive cells, reducing brain peroxidation injury: to improve the neurological symptoms. The clear solution Ecchymosis capsules confrontation effect on hemoglobin toxic effects of intracerebral hemorrhage in rats, and thus play the role of brain protection. The mechanism may be associated with reduced hemoglobin release of toxic substances, and enhanced brain tissue antioxidant capacity, reduce peroxidation, cerebral edema and other factors related. The results show that the clear solution Ecchymosis capsules can reduce the toxic effects of hemoglobin after intracerebral hemorrhage, suggesting Tongfu diuresis, cooling blood detoxification brain serum solution Ecchymosis reasonable treatment method for the treatment of hemorrhagic stroke from the poison theory provides the basis for .

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