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Objective: To investigate Rover extract Lasianthus duck hepatitis B treatment efficacy. Method: 1 using 70% ethanol extract Lasianthus Rover, Rover Lasianthus prepared ethanol extract. 2 Sun modified Karber method for the determination of its oral acute toxicity, calculated LD50. 3. Strong positive serum with DHBV DNA 0.2ml to 1-day-old ducklings injected intraperitoneally, 15 days later using fluorescence quantitative polymerase chain reaction (FQ -PCR method) screened positive for DHBV strong duck, duck hepatitis B virus infection in an artificial animal model. Stratified random allocation method to artificially infected with DHBV experimental animals were randomly assigned to five groups. Of which four treatment groups were given high doses Rover Lasianthus ethanol extract (2.86g/kg · day), the dose Rover Lasianthus ethanol extract (1.43g/kg · day), low-dose Rover Lasianthus ethanol extract ( 0.72g/kg · day) and positive control drug acyclovir (ACV) therapy, set a model for the control group, with double-distilled water instead of drugs, and then a separate one group received no treatment, DHBV was detected by PCR negative ducks as normal control group, also given the same amount of double-distilled water. N = 12 in each group of ducklings were fed for 14 days. Before treatment (T0), after administration of 7 days (T7), 14 days after treatment (T14), five days after discontinuation (P5) blood samples were collected, using the FQ-PCR assay of serum DHBV DNA load, using ELISA Determination of Serum DHBsAg OD values ??while serum transaminase (AST, ALT), animals were sacrificed livers final pathological examination. 4 Data analysis: DHBV DNA, DHBsAg, ALT and AST test data using repeated measures analysis of variance, the pathological effects of drugs on duck data by rank sum test for statistical analysis. Data processing and analysis are used statistical software SPSS13.0 for windows. Results: 1 to 2kg Rover Lasianthus crude drug preparation alcohol extract 302g, oral toxicity test indicates the LD50 for mice 22.91g/kg, 95% CI 21.41 ~ 24.51g / k (g Sun improvement Kou's method), are non-toxic level. (2) the observation period, each treatment group, only ACV group of viral load lower than the model group (P = 0.043), Description ACV could significantly inhibit DHBV replication, but the 14 days start to withdrawal five days, the viral load rebound occurs. Each treatment group and model group viral load trends over time are relatively close, the difference was not statistically significant (P gt; 0.05), Rover Lasianthus ethanol extract of high, medium and low-dose three treatment groups did not show significantly inhibit DHBV activity. 3 observation period, the normal group DHBsAg OD values ??were significantly lower than the treatment group and model group, the difference was statistically significant (P lt; 0.05); each treatment group compared with the model group, the difference was not statistically significant (P gt; 0.05), indicating that this experiment Rover Lasianthus ethanol extract of the three dose groups were not inhibited DHBsAg expression. 4 observation period, each treatment group and the model group point of ALT and AST was significantly higher than the normal group, the difference was statistically significant (P lt; 0.05); each treatment group compared with the model group, the difference was not statistically significant (P gt; 0.05), described this experiment Rover Lasianthus ethanol extract of the three dose groups were not reduced ALT, AST levels and improve liver function. 5 liver pathology examination found no animals in each group the severity of liver disease was significantly (P gt; 0.05), described Rover Lasianthus alcohol extract of three dose groups had no role in protecting the liver. Conclusions: 1. Rover ethanol extract Lasianthus high, medium and low dose groups showed no inhibition of DHBV replication role. 2 Rover ethanol extract Lasianthus high, medium and low dose groups showed no right DHBV induced liver injury in rats.
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