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Objective: To observe the influence of HXJZL on early AS rats’blood lipids, blood viscosity, oxidative stress-related factors and histomorphology, the study preliminary elucidate the anti-oxidative stress effect of HXJZL on early AS rats, provide experimental evidence for the future clinical application.Methods: 60 healthy male Wistar rats were randomly divided into 5 groups named Control group, Model group, Simvastatin group, HXJZL high dose and HXJZL low dose group, 12 rats in each group. The rats in Control group were fed with fundamental feed for 12 weeks. Model and each treated group were fed with high fat feed, and peritoneal injected with VitD3 at the dose of 700000U/kg which divided into 3 days after 4 weeks when the experiment began. Then saline were given to model group at 10ml/kg weight; suspension of simvastatin(CO: 1mg/ml) were given to Simvastatin group at 10mg/d/kg(equal to 20 times to clinical dose) weight, HXJZL high dose and low dose group were given HXJZL at the dose of 45g/d/kg(equal to 20 times to clinical dose) and 22.5g/d/kg(equal to 10 times to clinical dose) weight, medicinal broth’s CO were 4.5g/ml and 2.25g/ml.The time of intragastric administration last 8 weeks.After the last administration, anaesthetized all rats at the second morning when empty stomach, and then quickly collected rats’blood on the way of carotid arterial cannula to determine the count of TC, TG (oxidizing enzyme method), HDL-C,LDL-C (chemical modification method), NO (nitrate reductase method), SOD(xanthine oxidase method), MDA(TBA method), T-AOC(chemical shade selection method). HE staining was employed to analyze the pathological change in arteriae aorta and SP staining to detect the expression of iNOS in arteriae aorta.Results:1 Rats’general conditionThe rats in Control group were gloss-color pattern, active and honey stomach, whose weight increased gradually. After given to high fat fed, the Model group rats’weight increased obviously because of eating much. But as the experiment proceeded, the color pattern’s dimer, and the rats were less of activity. In midanaphase, the appetite didn’t increase and the speed of weight gain decreased. The rats’condition of Simvastatin group, HXJZLHigh, Low group was better to Model group. In early metaphase, the rats were active and honey stomach. Simvastatin group’s weight increased in the initial stage while not in midanaphase. HXJZLHigh, Low group rats’weight decreased a little in the initial stage after intragastric administration while increased gradually in midanaphase, both groups had no significant diference.2 The change of blood lipidsCompared with Blank group, the content of TG, TC, LDL in Model group increased obviously, HDL decreased, the difference was statistical significance (P<0.01).Compared with Model group, the content of TG, TC, LDL in Simvastatin group, HXJZLHigh,Low group all decreased obviously, HDL increased obviously, the difference was statistical significance (P<0.01). Compared with Simvastatin group, the content of TC in HXJZLHigh group increased, the difference was statistical significance (P<0.01), TG, HDL, LDL no difference (p>0.05); the content of TC, LDL in HXJZLLow group increased, HDL decreased, the difference was statistical significance (P<0.05), while TG was no difference (p>0.05).3 The change of blood viscosityCompared with Blank group, the content of blood viscosity in Model group increased obviously, the difference was statistical significance (P<0.01).Compared with Model group, the content of blood viscosity in Simvastatin group, HXJZLHigh,Low group all decreased obviously, the difference was statistical significance (P<0.01). Compared with Simvastatin group and HXJZLLow group, the content of blood viscosity in HXJZLHigh group decreased, the difference was statistical significance (P<0.01). The difference of blood viscosity was no statistical significance in Simvastatin group and HXJZLLow group (P>0.05). 4 The change of SOD, MDA, T-AOC, NOCompared with Blank group, the content of SOD, T-AOC, NO in Model group decreased obviously, HDL increased, the difference was statistical significance (P<0.01).Compared with Model group, the content of SOD,T-AOC,NO in Simvastatin group, HXJZLHigh, Low group all increased obviously, MDA decreased obviously, the difference was statistical significance (P<0.01). Compared with Simvastatin group, the content of SOD in HXJZLHigh group decreased, the difference was statistical significance (P<0.01), T-AOC increased, the difference was statistical significance (P<0.01). The difference of MDA, NO was no statistical significance (P>0.05); the content of SOD, NO in HXJZLLow group decreased, the difference was statistical significance (P<0.01), MDA increased, the difference was statistical significance (P<0.05), the difference of T-AOC was no statistical significance (P>0.05).5 The morphologic observation of arteriae aortaObviously pathological damage had not been seen in arteriae aorta of Blank group. In Model group, the tunica media and endomembrane were thickening, where many of macrophage and histoleucocyte infiltrated and hyperplasied accompanied with foam cells formed and calcified; there were a lot of collagenoblast hyperplasied in ectosome which structure were confused and incomplete. In Simvastatin group, there wasn’t obviously pathological damage in tunica media and endomembrane of arteriae aorta as cells to line up in order, however, the structure of tunica media was indistinct. In HXJZLHigh group, each layer of arteriae aorta was distinct and integrated, but a little cellular proliferation in tunica media and endomembrane. In HXJZLLow group, the endomembrane was a little thicke, SMC was not regular that had a few hyperplasy of fibrous tissue, the structure of ectosome was not distinct.6 Expression of iNOSCompared with Blank group, the expression (OD) of iNOS in Model group increased obviously, the difference was statistical significance (P<0.01).Compared with Blank group, the expression (OD) of iNOS in each treated group was decreased obviously, the difference was statistical significance (P<0.01). Compared with Simvastatin group, there was no statistical significance in HXJZLHigh group (P>0.05); iNOS in HXJZLLow group increased, the difference was statistical significance (P<0.05).Conclusion: HXJZL can regulate early AS rats’metabolism of lipids, degrade the value of blood viscosity, ameliorate the pathomorph of arteriae aorta, elevate the expression of anti-oxidative stress correlation factor, decrease the lipid peroxidation, accordingly play the role of degrading the oxidative stress damage and inhibiting the development of AS.
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