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Objective: hemorrhagic fever with renal syndrome in Hebei Province (HFRS) Fever in Human infection and mice carry hantavirus (HV) gene sequencing and comparative analysis by the DNASTAR software on both to clarify the mice of the same area to carry HV infection HV both etiological characteristics and connections to provide a scientific basis for the prevention and control of HFRS. : 1 through epidemiological surveys, collection of cases or suspected cases of acute phase serum specimens; carried out in the same area the night trapping catching mice, the sterile rat lung; using indirect immunofluorescence assay (IFA) screening positive serum samples, from extraction HV RNA; cryostat-cut rat lung cold acetone fixed, the IFA screening HV positive rat lung; 3 by reverse transcription-nested polymerase chain reaction (RT-nested PCR) amplification of viral nucleic acid, were genotyped type; positive rat lung extract HV RNA, reverse transcriptase cDNA synthesis, according to the gene sequences of the strains known HV standard design SEO and HTN-specific primers, was amplified by RT-nested PCR for genotyping; 4 Select representative infected areas serum samples and the same region rat lung specimens PCR products were sequenced after purification and recovery; DNASTAR software to infect humans HV and mice carrying HV homology and phylogenetic tree, on the same area of ??human infection HV and HV mice carrying compare. Results: an experimental serum samples collected cases or suspected cases 158, 101 IFA testing positive, the positive rate was 63.92%. Positive serum samples by RT-PCR, 17 positive results, the positive rate was 16.83%. 2 Hebei Province HV-positive patient serum samples at the same time with SEO and HTN genotyping primers RT-nested PCR amplification to display positive samples carried HV are SEO type of HTN is not found. Three experiments from a 1630 rat lung the positive rat lung 43 were infected was 2.64%. From across the province rat density and infection rate distribution, HFRS foci in our province infected rat Rattus norvegicus mainly in the residential areas, followed by residential areas Mus musculus, the wild infected mice is not the main source of infection. The positive rat lung specimens of total RNA with the G1 fragment SEO and HTN genotyping primers to amplify the positive samples carried HV are SEO type of HTN is not found. 5 people infected with HV mouse carrying HV G1, G2 fragment gene mutation analysis of the 5.1 HV infection with mice carrying HV G1 Nucleotide homology and genetic variation across the province as a whole human infection HV mouse carrying HV G1 fragment homology comparison showed G1 fragment of the nucleotide sequence homology as high as 95.0% to 99.7%. Wherein the SJZ and ffTS for 95.0%, 99.7% the CD2, BD1, BD2, BD3 and ffSJZ, QHD2, TS and ffQHD, CZ1, CZ2, and ffCZ are, the rest of between 95.2% to 99.4%. The same area two specimens were: from 95.5% to 96.4% in Baoding, Chengde 95.5% to 96.6%, and 99.7% in Cangzhou, and Qinhuangdao from 95.8% to 99.7%, and 95.2% in Shijiazhuang, Tangshan 99.4%. Nucleotide homology highest the CZ1 and ffCZ of of of Cangzhou the Qinhuangdao QHD2 and ffQHD are 99.7% nucleotide homology minimum for Baoding BD1 and ffBD1 and Chengde CD1 and ffCD1, 95.5% . Tip, HV is a high homology between the same area between the mouse and human, or in different regions of the mouse and human. 5.2 HV infection mouse carrying HV the G2 fragment nucleotide homology and genetic variation from the nine human serum and 7 rat lung specimens between the extraction G2 Nucleotide homology of up to 92.7 % ~ 99.7%. Wherein the CZ2 ffBD1 between the homology for 92.7% and 99.7%, respectively the CD2 and ffCZ, BD3, BD1 and ffSJZ QHD ffTS are the rest were between 93.7% to 99.3%. The same area two specimens were: Baoding 95.7% to 98.7%, from 98.7% to 99.3% in Chengde, Cangzhou 97.3% to 98.3%, and 94.7% in Shijiazhuang, Tangshan was 99.3%. The nucleotide homology highest for of Chengde CD2 and ffCD1 and Tangshan TS and ffTS are 99.3% to 94.7%, of nucleotide homology minimum Shijiazhuang the SJZ and ffSJZ. G1 (217-573nt) and G2 (2002-2301nt) fragment phylogenetic tree was constructed using DNASTAR package. G1 fragment constructed phylogenetic tree display the QHD, ffQHD, TS, ffTS strains kinship close. The close kinship ffBD1 Sjz strains, the CZ1 ffCZ strains closer relationship. G2 fragment constructed phylogenetic tree display ffSJZ BD1, BD3 strains of kinship close QHD2, TS, FFTS and strain genetic relationship close SJZ, ffBD1 strains kinship close, which is more consistent with the G1 fragment, indicating that the two homologous relatively close. Other sequence homology farther. Seven genotyping and phylogenetic tree analysis showed that Hebei Province is a typical SEO endemic areas. Genetic subtypes in accordance with the G1, G2 fragment is divided into S1, S3 two subtypes, typing results are S3 main subtypes found no new subtype. In addition to the Chengde Mus musculus carried HV phylogenetic tree was constructed according to the G1 fragment typing S4 subtype, G1 and G2 fragment genotyping results are basically the same. Conclusion: 1 Whether the same area, or different regions, Hebei Province, People infected with HV mouse carrying HV highly homologous to, Rattus norvegicus carry HV is the most important source of HFRS patients infected. 2 Hebei Province of human infection with HV SEO-based, including a total of two subtypes of S1, S3, S3 subtypes. Foci host animal carried HV genotype SEO type the genetic subtypes S3 subtypes, and is widely distributed in Hebei Province. 3 in the geographical proximity of the region, the viral genome nucleotide sequence homology is relatively high, closer phylogenetic relationships, the HV distribution has a certain geographical regional.
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