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Effect of Rh-Endostatin on the Expression of ERCC1 in Human Lung Adenocarcinoma A549 Cells

Author: YanYan
Tutor: SunXiuHua
School: Dalian Medical University
Course: Oncology
Keywords: Lung Cancer Recombinant human endostatin Cisplatin ERCC1 A549 cells
CLC: R734.2
Type: Master's thesis
Year: 2011
Downloads: 26
Quote: 0
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Lung cancer is gradually increased in recent years, the incidence of malignant tumors of various drug phenomenon greatly limits its clinical efficacy, so the genes involved in resistance and its mechanism of action will be to a large extent for the treatment of lung cancer to help. Cisplatin (DDP) is the clinical treatment of lung cancer one of the most commonly used chemotherapy drug, platinum-based drugs through the induction of tumor cell DNA damage caused by DNA replication disorder, and DNA repair systems can repair DNA damage caused by drugs, so that the body to produce anti- potency. The present study showed, ERCC1 gene expression and DDP resistance phenomenon intrinsically linked. Recombinant human endostatin (rh-Endostatin), trade name Endostar (Endostar), is China's first independently developed anti-angiogenic drugs, which in combination with chemotherapy drugs can increase the efficacy of chemotherapy, and this drug fewer side effects, good security. The experiment will be DDP, Endostar different modes of administration role in the human lung adenocarcinoma A549 cells, the drug treated cells mRNA expression of ERCC1 gene analysis. Objective: To investigate Endostar and DDP different modes of administration of human lung adenocarcinoma A549 cells ERCC1 gene expression, in order to provide theoretical guidance for second-line treatment of lung cancer. Methods: Human lung adenocarcinoma cell line A549 as the research object, the use of drugs for the DDP and Endostar. Were detected by MTT Endostar, DDP and DDP combination Endostar on A549 cell growth inhibition, and to determine the concentration of the drug used in the experiments. Logarithmic growth phase of the experiment were divided into four dosing groups, a control group, were: (1) DDP added in one (0.34μg/ml); (2) DDP minutes three days added (0.11 per day μg / ml); (3) DDP (0.34μg/ml), Endostar (300μg/ml) was added in one; (4) DDP minutes three days added (daily 0.11μg/ml), while added daily Endostar (300μg/ml ); (5) the control group. The grouping method according to the relevant drugs act on the cells, using Real Time PCR Detection of the treated cells relative expression of ERCC1mRNA. Results: 1.MTT method to get Endostar when the concentration of 300μg/ml, on A549 without significant cytotoxicity. DDP on A549 cells IC 30 is about 0.34μg/ml. When combined Endostar DDP on A549 cells IC 30 is about 0.13μg/ml. 2.Real Time PCR method derived cells ERCC1mRNA each experimental group relative expression levels were: ① 1.00; ② 0.88; ③ 2.00; ④ 1.90; ⑤ 0.77. Accordingly drawn: (1) dosing group were higher (2) were higher than the United Endostar DDP alone group. Conclusion: 1.Endostar increased DDP on human lung adenocarcinoma A549 cell cytotoxicity. 2.DDP and Endostar combination makes ERCC1mRNA A549 cells was significantly increased.

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CLC: > Medicine, health > Oncology > Respiratory system tumors > Lung tumors
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