Dissertation > Excellent graduate degree dissertation topics show

Studies on the Screening of a Lipopeptide Biosurfactant Producing Bacterium and Properties of the Lipopeptide

Author: QianXiaoYong
Tutor: YeMing；LiuGuoQing
School: Hefei University of Technology
Course: Processing and storage of agricultural products
Keywords: Biosurfactants Screening condition optimization Physical and chemical properties Safety evaluation
CLC: Q93
Type: Master's thesis
Year: 2009
Downloads: 245
Quote: 3
Read: Download Dissertation

Abstract


This paper studied the screening of a biosurfactant,the properties and the safety evaluation for application.The main results were as follows:1.A series of bacteria were isolated from soil samples and water samples from Hefei University of Technology,Hefei oil storeroom and Dongying Victory scum pool from Shangdong province by a series of steps including hydrocarbon enrichment culture,hemolytic activity assay on blood agar plates and surface activity assay etc.One strain was purified and was named as H-02.It is identified as a Bacillus subtilis by biophysical and biochemical experiments according to《Bergey’s Manual of Determinative Bacteriology》,16S rDNA sequence analysis. The biosurfactant produced by strain H-02 was founded as a lipopeptide biosurfactant.It has a satisfacting activity with a oil displacement diameter at 9.2 cm.Furthermore the emulsion of this lipopeptide is fine as well for maintain the emulsion for at least 10 days.2.The condition for lipopeptide production was optimized by single factor experiments and orthogonal test.Results were as follows（g/100mL）:soluble starch 2.5,NH4NO3 0.6,MgSO4·7H2O 0.04,CaCl2 0.008,yeast extract powder 0.08, KH2PO4 0.24,Na2HPO4 0.96,pH 7.0-7.5.At the inoculum of 5.0%,under 35℃, the yield of lipopeptide was 3.18 g/L.3.The physical and chemical properties of lipopeptide produced by strain H-02 was investigated.Results showed that this biosurfact produced by H-02has a excellent emulsion and vesicant characteristic,and anti-microorganisms traits just like antibiotics.So it could be used for many domains as MEOR,Food and Pharmacy. Also this lipopeptide can maintain its activity under serial extreme conditions like high temperature,high concentration salt,especially Ca2+ cation,and low pH.The suitable pH was identified between 4.0 and 10.0.4.The acute toxicity test and thirty days feeding study was performed,results showed that the lipopeptide has no obvious toxicity to little mouse.

Related Dissertations

  1. Establishment of the Molecular Identifying System of Curvualria and Application in Difficult Species,Q949.32
  2. Identification and Lipopeptide Analysis of Biocontrol Bacillus Spp. Isolated from Tibet,S476.1
  3. Comprehensive Evaluation of Safety Production and Optimization of Safety for Yangcheng Coal Mine,X936
  4. Strain Screening of Hainanmycin Production and Fermentation Process Optimization,TQ927
  5. The Design of the Coal Mine’s Safety Evaluation System Based on Wavelet Neural Network,TD79
  6. Efficacy and Safety Evaluation of Claritin Treatment on Children with Asthma,R725.6
  7. Research on Fast Predefined Contingency Screening Method of Power System Transient Stability,TM712
  8. Fosmid library and PCR -based screening technologies spider silk protein gene package egg and Analysis,Q78
  9. Analysis on the Indicator Thresholds of Roads’ Grade Length and Gradient in Xinjiang Main Roads Safety Evaluation,U412.3
  10. Environmental and ecological security evaluation system based on BP neural network city ranks,TP183
  11. Establishment Andapplication of Two in Vitro Screening Methods for Cosmetic Additives with Anti-aging Effect,TQ658
  12. Establishment and Evaluation of a Murine Nephritis Model with Chronic Graft-versus-host Disease,R392
  13. Study on Phylogenetic Diversity and Bioactivity of Symbiotic Fungi of Coral,R284
  14. Polysaccharides from fecal Coprinus mycelium , structural analysis , and inhibition of S-180 tumor,R285
  15. The Research of Nutritional Risk in Hospitalized Patients of Gastroenterology in Meizhou,R57
  16. Experimental study of anti- metabolic syndrome drug screening and DA anti- metabolic syndrome, the role and mechanism of action,R589
  17. Optimising the Screening Model for High Risk Pregnancy,R715
  18. Accuracy of FISH for 3q26 Gains as a Screening Marker in Cervical Cancer,R737.33
  19. The Role of CAT25 in Screening Hereditary Nonpolyposis Colorectal Cancer,R735.34
  20. TCT, DNA Imaging Cytometry and HPV Testing for Cervical Cancer in Xinjiang Uygur Census Research,R737.33
  21. The Study and Application of Enzyme Biochromatography for the Screening of Aminopeptidase N Inhibitors,R730.2

CLC: > Biological Sciences > Microbiology
© 2012 www.DissertationTopic.Net  Mobile