Dissertation > Excellent graduate degree dissertation topics show

20-HETE-Induced Changes in Inotropic Effects Depend on the Activation or Inactivation of L-type Calcium Channels by PKA and\or PKC in Rat Ventricular Myocytes

Author: LiXingZuo
Tutor: ZengQingHua
School: Northeast Normal University
Course: Physiology
Keywords: 20-HETE Ventricular myocytes L- type calcium channel currents Intracellular calcium ion concentration Calcium transient PKA PKC
CLC: Q26
Type: Master's thesis
Year: 2009
Downloads: 55
Quote: 0
Read: Download Dissertation

Abstract


Cytochrome P450s (CYP) ω- hydroxylase arachidonic acid metabolites 20 - arachidonic acid hydroxyalkyl eicosane (20-HETE), has an important role in regulation of vascular tone , but the 20-HETE on the heart the role has rarely been reported . We studied the effects of exogenous 20-HETE on myocardial contractility regulation and its mechanism. Using the isolated perfused Langendorff systematically studied 20-HETE on isolated cardiac effects of myocardial mechanics , found that low doses of 20-HETE (0.1-3 nmol / L) on the perfused heart has a positive inotropic effect , whereas higher doses ( 10 nmol / L) is a clear negative effect. Then we use Fura-2/AM fluorescence measurement and patch clamp techniques to study the 20-HETE in rat ventricular myocytes isolated intracellular calcium concentration ([Ca2] i) and L- type calcium channel effect. We found that low doses of 20-HETE (0.01, 0.03, 0.1μmol / L) increased in a dose -dependent manner [Ca2] i, and high doses of 20-HETE (0.3, 1μmol / L) induced [Ca2] i in reduced. And that low doses of 20-HETE (0.1μmol / L) induced by whole cell L- type calcium channel current amplitude increased, while the high dose of 20-HETE (1μmol / L) has the opposite effect . With a laser scanning confocal microscope line function testing 20-HETE on intracellular calcium transients , found that low concentrations of 20-HETE (0.01,0.1 μmol / L) can increase the amplitude of calcium transient and prolonged calcium transient decline phase schedule ; high concentrations of 20-HETE (1.0μmol / L) can reduce the calcium transient amplitude and prolong the time course of calcium transients . The 20-HETE -induced [Ca2] i, whole-cell L- type calcium channel currents , calcium transient increase in the amplitude of such effects may be protein kinase C (PKC) inhibitor chelerythrine base and cAMP-dependent protein kinase (PKA) inhibitor H-89 block. PKC and PKA activity measurement results show that 20-HETE (0.03μmol / L) increased cardiac activity of PKC and PKA , and the red base celandine (5μmol / L) , and H-89 (5μmol / L) inhibited this effect . These data suggest that 20-HETE may activate or inhibit L-type calcium channels to regulate [Ca2] i, thereby adjusting the force of myocardial contraction , and these effects mediated by the PKC and PKA .

Related Dissertations

  1. Naloxone on rat ventricular myocytes L-type calcium channels,R965
  2. CX43, PKC and HSP70 in cardiac sudden death by cardiac carcinoma and its significance in forensic medicine,R541
  3. Different to Stroke Hemiplegia Supersession Acupuncture Rats Spasticity PKA Content of Impact Study,R246
  4. An Investigation on the High PKA Activity and Heat Shock Resistance of Saccharomyces Cerevisiae,Q932
  5. Acid- sensitive ion channels in the myocardium on the expression and distribution of its electrophysiological properties,R96
  6. The Ion Channel Mechanism of Inotropic Effect of Apelin-13 on Rat Heart,R33
  7. The Effect and Ion Channel Mechanism of Hydrogen Sulfide on Rat Heart Function,R363
  8. Role of PKA in NMDA Receptor-induced NMDA Receptor Synaptic Accumulation in Spinal Dorsal Horn during Inflammatory Pain,R96
  9. Role of the CaMKⅡ/PKA Signal Transduction Pathways in Generation of Triggered Arrhythmias in Chronic Heart Failure,R541.6
  10. Experiment the Affects of "PKC、 MMP-9 of Lung Tissue" of Asthma Rats on "Ma Xing Xiong Ting Combination",R285.5
  11. Radiation Damage in BaTiO3 Investigated by Computer Simulation,O614.411
  12. The Electrophysiologic Study of Taurine Magnesium Coordination Compound on Abnormal Ion Channel Induced by Hypoxia/Reoxygenation in Cardiomyocytes of Rat,R96
  13. Expressions of 5-HT—5-HT2A R—PKC Pathway in Hypoglossal Nucleus in Normal Rats,R56
  14. Studies on Content of PKC by Acupuncture in Different Meridian Points of Stroke Hemiplegia Spasticity Rat,R245
  15. Effects of Visfatin on Ion Channels in Rat Cardiac Ventricular Myocytes,R54
  16. Effects of 20-HETE on Intracellular Ca2+ Overload Via NADPH Oxidase-derived Superoxide Production in Neonatal Rat Cardiomyocytes,Q46
  17. BK Influence on 20-HETE Induced Changes on Myocardial Mechanics on Rat,Q4
  18. Study of 20-HETE Induces CardiacMyocytes Apoptosis Through Mitochondrial-dependent Pathways,Q46
  19. Cytochrome P450 ω-hydroxylase Inhibition Attenuates Isoprenaline-induced Apoptosis in Cultured Myocardiocytes,R96
  20. The Effects of Glucagon Like Peptide-1 Against the Injury of Oxidative Stress Induced by High Glucose and Its Mechanism,R541
  21. EA \,R245

CLC: > Biological Sciences > Cell Biology > Cellular Biochemistry
© 2012 www.DissertationTopic.Net  Mobile