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Studies on Nano-modified Amperometric Enzyme Biosensor and Its Application in Pesticide Residue Analysis

Author: ChenZuoJuan
Tutor: DuDan
School: Central China Normal University
Course: Analytical Chemistry
Keywords: Nano Decorate Acetylcholinesterase Organophosphorus hydrolase Carbamate and organophosphate pesticides Biosensor
CLC: X839.2
Type: Master's thesis
Year: 2009
Downloads: 195
Quote: 2
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Abstract


Since the discovery of the enzyme biosensor has been highly selective , simple structure , automatic , inexpensive attracted the attention of researchers . Microelectronics technology , the development of nanofabrication technology , bio- technology to extend the range of applications of biosensors , mass production , integration , miniaturization has laid a solid foundation , and greatly contributed to the research and application of enzyme biosensors . The enzyme - based biosensors have been widely used for the detection of pesticides , in particular, is based on the catalytic activity of acetylcholinesterase (AChE) inhibitory enzyme electrode and the degradation of organophosphorus pesticides based on direct enzyme organophosphorus hydrolase (OPH) electrodes have been widely used for the detection of organic phosphorus . The enzyme immobilization technology is the key step of the preparation of a biosensor which will directly affect the stability of the sensor , sensitivity , detection limit , the response time and the activity of the enzyme , the survival time . Enzyme immobilization technology mainly including the choice of the carrier material of the enzyme immobilization method and the fixed enzyme . At home and abroad for enzyme immobilization technology in biosensors extensive research and made ??important progress . This thesis, on the one hand to build the current type of nano- modified acetylcholinesterase biosensor for the detection of carbamate and organophosphate pesticides analysis ; On the other hand , the current type organophosphorus hydrolase biosensors constructed nano -modified analysis for organophosphorus pesticides detection . In short , we build acetylcholinesterase and organophosphorus hydrolase biosensors can be widely applied to the detection of pesticides and can be used to study drug and pesticide sensitive .

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CLC: > Environmental science, safety science > Environmental Quality Assessment and Environmental Monitoring > Environmental monitoring > Monitoring of agricultural chemicals
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