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Establishment the Characteristic Macrorestriction Map of Common Red-tide Algae by RFLP

Author: ZhangTao
Tutor: YuZhiGang；MiTieZhu
School: Ocean University of China
Course: Ecology
Keywords: red tide rDNA RFLP
CLC: X173
Type: Master's thesis
Year: 2009
Downloads: 137
Quote: 2
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Abstract


The red tide causes serious damage to the inshore environment of our country. Therefore,it becomes more and more important to prevent and control the red tide.It is a critical and fundamental job to identify the red tide algae.With the development of molecular biology,molecular biological technology applied to taxonomy becomes more and more popular and matured.The method for classification and identification of the red tide algae by its ribosome DNA sequence based diversity attracts more and more attention both at home and abroad due to its easy operation and fast characteristics.In this paper,PCR-RFLP was used to construct the characteristic macrorestriction map of main species of red tide algae,and based on this,an efficient identification method based on RFLP was established.The result comprises two aspects:(1)We acquired 18s rDNA segments of 20 species of red tide algae with the PCR technology, and optimized the experimental condition of PCR.(2)18s rDNA segments of 20 species of red tide algae were analysed by RFLP,and the result shows that obvious distinction exists in 20 species of red tide algae.We set up the characteristic macrorestriction map for main species of red tide algae and developed a method for efficient and quick identification of red tide algae based on RFLP.By effectively identifying the red tide algae,a new method was establishd for pre-warning,prevention and control,and assessment of the red tide.Meanwhile,it provide a new technology for the study on biodiversity of the oceanic phytoplankton.

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