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In this study, first studied two fungal toxin detection method , using immunoaffinity column purification deoxynivalenol in Fusarium enol malting barley (Deoxynivalenol, DON) Detection of quantitative methods using high performance liquid chromatography . Standard added to the malting barley the spiked range between 0.10-2.00μg / g , the recoveries were 92.04% -97.29 % , the relative standard deviation of 0.92% -2.67 % . Detection limit 0.01μg / g , while some samples for testing, the measured sample content in the range of DON between 0.0123-0.0926μg / g . Using immunoaffinity column purification using HPLC quantitative method of malting barley ochratoxin A (Ochratoxin A, OTA) detection . Standard added to the malting barley the spiked range between 0.1-10.0μg/kg , recoveries of 93.15% -97.07 % 0.54% -2.19% relative standard deviation , detection limit 0.05μg/kg . Successfully established two methods on the basis of the experimental full range of tracking determination of the brewing process DON and OTA , DON and OTA in various process changes , and its appropriate physical and chemical indicators of malt and beer . Studies have shown that DON and OTA steeping process has suffered serious losses , DON loss of 88.6% , a loss of 87.7% and the OTA , DON and OTA content increase in germination and saccharification process , drying , boiling and fermentation process hardly changes. In addition , DON and OTA will affect the germination rate of barley to produce enzymes and malt extract reduced the Chur Bach values ??reduce the viscosity increases , diastatic power decreases . Variety of detoxification methods , the final ozone detoxification , ozone machines produce ozone , processing spiked malting barley , the ozone concentration of 30 mg / L , the processing time of 60 min , the detection of the treated samples by liquid chromatography . The results showed that the detoxification effect , there is no chemical residues , and will not affect the germination rate of barley .
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