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Search Inhibitor of 5α-reductase in Brassica Napus L. Pollen

Author: SunHong
Tutor: LiuHeGang
School: Hubei College of Traditional Chinese Medicine
Course: Of Pharmacy
Keywords: Rape pollen 5α-reductase Inhibitors
CLC: TQ464
Type: Master's thesis
Year: 2009
Downloads: 69
Quote: 0
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Abstract


Prostatic hyperplasia is a common disease of middle-aged men frequently occurring chemical drugs have some side effects , and proprietary Chinese medicines Qianliekang listed since efficacy is clear , no significant side effects , so depth study of the mechanism of Qianliekang separation structures were identified by analysis of its active ingredient , the active ingredient , it is possible to discover new lead compounds , and thus lay the foundation for new chemical substances not found . The main ingredient Qianliekang rape pollen . For the inhibition of 5α-reductase , in the mechanism of action is written in the specification of the Qianliekang conversion of testosterone to dihydrotestosterone , the role of chemical composition but which have failed to reveal . Firstly established analytical method , in comparison liquid chromatography , ultraviolet spectrophotometry several enzyme activities , an activity analysis method based on gas chromatography , disappeared with the product of the substrate testosterone were measured dihydrotestosterone generate speed , specificity , and reproducibility . This way , and then the rape pollen extract different components were added to the enzyme reaction system , inhibitory effect was measured in order to suppress the active ingredient in the rate indicators Filter rape pollen . The enzyme extracted from the rat testis tissue , dispensing and freeze-dried preservation TYPE Ⅰ and type two subtypes II 5α-reductase exist , two subtypes pH5 and pH7 environment showed maximum activity , and the TYPE Ⅱ closer relationship with benign prostatic hyperplasia , and therefore choose tYPE Ⅱ 5α-reductase reaction system . Experimental studies have shown that the active ingredient found in the ethanol extract of the rape powder after silica gel column chromatography to give a yellow oily substance , and again confirmed the in vitro inhibitory activity . The purity checks this yellow oily substance by a single compound, identified by GC-MS, NMR , and it is a multi- polyether having a molecular weight of 456 , the Formula C 28 H 56 < / sub> O 4 . Enzyme inhibition constants of Ki = 0.125, Ki = 0.098 same method measured the chemical drug finasteride . Rape pollen , there are a variety of trace elements, have enzyme inhibition . Limited time and funding limitations , can not be isolated and identified one by one , the article laid the foundation for an in-depth study of rape pollen .

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