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Objective: To compare the immediate implant, bone graft delayed planting and natural healing delayed implant osseointegration after different periods in different circumstances, for clinical choose three cropping patterns provide experimental basis. MATERIALS AND METHODS: dogs are divided into immediate implant group, three months after grafting delayed implant group and three months after the natural healing delayed implant group. Unplug dogs under anesthesia mandibular third and fourth premolars, get four extraction sockets, randomly select one of the three, according to the requirements of each implant. Three months after surgery, respectively, at planting, half of each group were sacrificed six dogs, drawing, fixed. Sacrificed before the trip tetracycline, alizarin red color fluorescent marker. Specimens general observation; imaging observation; doing with implant bone grinding, fluorescence microscopy implant - bone interface new bone osteogenesis; calculated using the two-color fluorescent labeling of each group of new bone formation around the implant rate; bone grinding staining underwent conventional optical microscope, measuring each group implant - bone contact rate (BIC) and peri-implant bone area (BA). Finally, the statistical software SPSS17.0 The data were statistically analyzed. Results: First, the general observation experimental animals with no deaths. In this study, a total of 72 implants, A1 group two month after implant loosening in the surrounding gum swelling, inflammatory granulation tissue curettage combined chlorhexidine-free remission after treatment in two out surgery. The remaining 70 implants were no infections, wound healed well after removal of soft tissue, implants grow well, and no loosening. Two, imaging observations X-ray examination, B1 group, a coronal implant has a depth of about 0.5cm wedge density to reduce the area, the surrounding bone becomes slightly dense, clear boundary. Residual implant - bone interface without significant transmission region, peri-implant bone density is normal. Second, the hard tissue biopsies for histological examination: six experimental groups implant and bone tissue have gained a better osseointegration, bone - implant contact rate (BIC) and peri-implant bone area (BA) were : BIC: A1 group, 69.46 ± 10.91; A2 group 78.54 ± 9.20; A3 group 57.33 ± 8.88; B1 group, 79.77 ± 13.12; B2 group 84.46 ± 6.44; B3 group 77.40 ± 8.36; BA: A1 group, 61.85 ± 6.82; A2 group 69.52 ± 9.63; A3 group 51.59 ± 7.00; B1 group, 73.25 ± 10.22; B2 group 77.47 ± 9.88; B3 group 69.22 ± 10.32. The statistical analysis showed that: BIC: p (A2 and A3), p (A1 and A3), p (A1 and B1), p (A3 and B3) were less than 0.05, statistically significant, the other was not statistically significant compared ; BA: p (A2 and A3), p (A1 and A3), p (A1 and B1), p (A3 and B3) were less than 0.05, statistically significant, the other was not statistically significant compared. Three, two-color fluorescence: Determination of six two-color fluorescence-labeled experimental group the distance between the strips, for statistical analysis, two-color fluorescent strip distance and the average mineralization rates were: two-color fluorescent marker spacing: A1 group, 6.13 ± 0.66 ; A2 group, 6.80 ± 0.43; A3 group, 5.23 ± 0.37; B1 group, 7.63 ± 0.61; B2 group, 8.38 ± 0.84; B3 group, 6.79 ± 0.54; average mineralization rate: A1 group, 6.13 ± 0.66; A2 group, 6.80 ± 0.43; A3 group 5.23 ± 0.37; B1 group, 7.63 ± 0.61; B2 group, 8.38 ± 0.84; B3 group 6.79 ± 0.54. The statistical analysis showed that: p (A2A3), p (A1A3), p (B2B3), p (B1B3), p (A1B1), p (A2B2), p (A3B3) were less than 0.05, statistically significant, other comparison was not statistically significant. Conclusions 1. Either immediate implant, bone graft delayed planting, or natural healing delayed planting, can achieve good osseointegration. Over time, increasing the probability of bone. (2) extraction socket bone after implantation of Bio-oss planting, implants and bone tissue can still be a good osseointegration and bone meal as Bio-oss bone induction, bone graft implant group than in the natural healing of bone after implant group combined rate. 3 bone - implant interface, bone remodeling activity at six months than three months when active.
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