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Study for the Absorbing Rules of Natural Passive Antibody (IgG) in Neogenesis Cow

Author: ZuoHaiYang
Tutor: HeZhaoYang
School: Jilin Agricultural University
Course: Preventive Veterinary Medicine
Keywords: Natural passive immunity Colostrum Calves ELISA
CLC: S823
Type: Master's thesis
Year: 2008
Downloads: 81
Quote: 1
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Abstract


The ruminant placenta connective tissue chorionic maternal antibodies can not be transferred to the fetus. Must be obtained by sucking colostrum to these animals, young animals born after natural passive immunity. Mainly includes the following process: before delivery, the cows will be established through the intestinal environment of a variety of suitable antibody selected transfer and enriched to the colostrum, postpartum colostrum containing a large number of immunoglobulin (Ig) secretion, calves by sucking colostrum, after the completion of the non-specific absorption of Ig in the gut into the blood circulation, thus avoiding the attacks of the newborn animal is a variety of pathogenic microorganisms. Reduce infection by milk prevention newborn animal, a way to prevent the disease, also called milk the immune (Lactogenic Immunity,). Immunoglobulins in colostrum more than 60% of the total whey protein, 80% IgG, the main anti-infection immunity of the animal's body, and also the serological study antibodies. Foreign reports can suck colostrum IgG levels calf serum in the period of time as a measure of the calf passive immunity transfer indicators. In this study, colostrum and calf serum IgG concentration variation was detected by ELISA, research methods and reference for the further study of passive immunization. In this study, saturated ammonium sulfate salting crude extracts from bovine serum IgG, the crude product over-glucan G 2 of 00 gel chromatography column of IgG and IgM hybrid protein, a few fiber Prime DEAE-52 column purified bovine IgG, IgG by SDS-PAGE electrophoresis identification. Rabbits immunized with a preparation of rabbit anti-bovine IgG antibodies, and preparation of rabbit anti-bovine IgG ELISA antibody labeled with horseradish peroxidase. The same time, the the immune mice Preparation of mouse anti-bovine IgG package antibody. Were established detection Colostrum and Calf Serum IgG antibody sandwich ELISA method. ELISA operating procedures: mouse anti-bovine IgG package carbonate buffer solution (pH9.5) was diluted to the optimum packet concentration 30μg/mL, respectively lessons of 100μL package reaction plate, and each plate Overcast positive control, an antibody control, control of the enzyme-labeled antibody and the substrate control, and incubated 3H is set 37%, the rejection to the package is liquid, as a washing solution containing 0.05% Tween-20 0.01mol/LpH7.4 PBS (PBST) washed three times, each time 3min (i.e., 3 × 3 'washing); be seized to be detected plus 4-fold dilution of colostrum of 100μL or a 100-fold dilution calf serum, 37 ℃ sense for 1.5h, 3 × 3' washed; enzyme-labeled antibody was diluted with 0.1% BSA-PBST to working concentration 1:400, 100 μL per well, 37 ° C sense for 1h, 3 × 3 'washed; added to each well of freshly prepared substrate (OPD) solution 100μL, 37 % wet box dark sense for 30min; each well 2mol/LH 2 SO 4 drop of the termination reaction, OD values ??determined by enzyme-linked immunosorbent assay (490nm) determination results. Siping area of ??a large dairy farm within one month of the trial design collection childbirth 20 cow colostrum and newborn calf serum. The application created colostrum and calf serum double antibody sandwich ELISA method for detection. The results show that: 1 after childbirth, the protein content of colostrum IgG from 0h start to seven days, changes showing a certain regularity. Fed colostrum calf serum IgG levels after a certain regularity. Performance from birth serum IgG is close to zero, and gradually increased, and 36h after birth, reaches the maximum, and after maintained at that level, and slowly decreasing trend with time. Control group calf serum IgG levels has been maintained at a low level.

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CLC: > Agricultural Sciences > Livestock, animal medicine,hunting,silkworm,bee > Livestock > Cow
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