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Hosta Liliaceae perennial herb, is the important flower foliage overcast habitat plants, more and more widely in the modern garden design. In this paper, the introduction of the 'gold standard' Hosta (Hosta'GoldStandard '), Hosta' Golden Delicious' (Hosta'Grand Tiara '),' mountain ridge 'Hosta (Hosta'Middle Ridge'), white flowers Hosta (Hosta plantaginea) and Northeast Hosta (Hosta clausavar.ensata) as test material, analysis of the root membrane permeability, with two artificial low processing Hosta soluble sugar, soluble protein, free proline and malondialdehyde and other physiological indicators of natural low temperature in winter variation, combined with the growth of the low temperature stress recovery observed a comprehensive evaluation of cold resistance of Hosta. Hosta application in Harbin feasibility and availability as a breeding germplasm to provide theoretical references. In the same time, the study also made a preliminary study of growth and development and propagation technology introduction Hosta The main results are as follows: (1) five hosta can grow normally in the Harbin area, and exhibit excellent ornamental characteristics. 'Golden Delicious' Hosta mosaic characteristics, ornamental characteristics. (2) two low-temperature treatment, hosta roots electrolyte leakage increases with decreasing temperature and low-temperature extension of the duration and amplitude of different kinds of Hosta electrolyte leakage increased. The artificial low processing relative conductivity curve combined logistic equation, obtained by the introduction of the 'Golden Delicious' Hosta 'gold standard' Hosta, white flowers, hosta, Northeast, Hosta, 'mountain ridge' Hosta roots LT50 - 22.65 ℃, -23.14 ℃, -20.47 ℃, -27.71 ℃, -25.78 ℃. (3) in artificial low process, with the extension of the duration of the decrease of temperature and low-temperature, Hosta root soluble sugar, soluble protein and malondialdehyde content, showed the first increase after reduction trend, but the increase rate between different species vary. Hosta roots of free proline content changes the overall trend is increasing, but the free proline change with temperature as well as the continuous low temperature processing time there is no clear correlation. (4) at the the two cryogenic treatment, with the prolongation of the duration of the decrease of temperature and cryogenic the type of the species Hosta Growth recovery survival rate decreased. Five Hosta cold resistance as follows: northeast Hosta 'mountain ridge' Hosta 'Gold Standard' Hosta 'Golden Delicious' Hosta Hosta white flowers. (5) different Hosta different parts of the explants callus induction, axillary buds, the upper bracts stems, pedicels, ovary induction rate is high, the material of choice is Hosta tissue culture propagation explant. 'Golden Delicious' the proliferation of hosta buds, stems and pedicels best medium is MS 6-BA 4.0mg / L NAA 0.2 mg / L; 'Golden Delicious' Hosta ovary bud proliferation medium MS 6-BA 3.0mg / of L NAA0.2 mg / L; optimum rooting medium was 1/2MS activated carbon 1g / L.
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