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The Study on Two Kinds of Molecular Marker among the Different Species of Cerasus Humilis(Bge)Sok and the Genetic Variation of Nutrition Indices

Author: WangZuo
Tutor: AoHong
School: Northeast Forestry University
Course: Genetics
Keywords: Cerasus humilis(Bge)Sok the SRAP molecular marker the ISSR molecular marker the soluble sugar the soluble protein
CLC: S662.5
Type: Master's thesis
Year: 2008
Downloads: 60
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Abstract


In this study,the trial materials were eight kinds of Cerasus humilis(Bge)Sok varieties from the Beijing Bureau of Forestry seedlings station.The Cerasus humilis(Bge)Sok were detected that the content of the soluble sugar and soluble protein had changed in different time.The optimal SRAP-PCR and ISSR-PCR system of the Cerasus humilis(Bge)Soke were first established in this research.Moreover,the ISSR molecular marker was applied to study the genetic diversity of the Cerasus humilis(Bge)Sok.The main findings were as follows:The analytic result from eight varieties of the Cerasus humilis(Bge)Sok showed that 61 polymorphism sites can be amplified from a total of 80 individuals by 11 primers,the proportion of the polymorphic loci was 78.21%,the average of each primer over 5.5 polymorphic sites.The genetic diversity of the eight kinds of Cerasus humilis(Bge)Sok was estimated by Shannon indice:Tangshan＞Huairou-labagoumen＞No.5 of Nongda＞No.4 of Nongda＞No.3 of Nongda＞Huairou-tanghekou＞Yanqingjianjiadian＞Huangshandian.There existed certain genetic differentiation in Cerasus humilis(Bge)Sok.The genetic variation among the populations was 25.32%of the total,and the other 74.68%distributed among individuals within populations.This indicated that the variation of Cerasus humilis(Bge)Sok mainly came from within populations.On the basis of the cluster analysis of genetic distance matrix,the eight kinds of Cerasus humilis(Bge)Sok can be divided into two categories:the genetic similarity among the varieties of Huairou-labagoumen、Yanqingjianjiadian、Huairoutanghekou、No.3 of Nongda,No、4 of Nongda and No.5 of Nongda are obvious;while the variation between Tangshan and Huangshandian were enormous with the others.There is remarkable difference in the soluble sugar content among the different Cerasus humilis(Bge)Sok varieties.In this study,the soluble sugar content of Tangshan and No.3 of Nongda are higher than other kinds of them at different periods,while the No.4 of Nongda and Huangshandian are lower.Similarly,the result also was conform to the soluble protein content in different times.The soluble protein content was higher,namely,Tangshan and No.3 of Nongda,and No.4 of Nongda and Huangshandian was lower.So,Tangshan and No.3 of Nongda were preferable varieties according to the soluble sugar and soluble protein content in different kinds of Cerasus humilis(Bge)Sok.

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