Dissertation > Excellent graduate degree dissertation topics show

Genetic Analysis and Molecular Marking of Resistance of Tobacco Cucumber Mosaic Virus

Author: FanJingYuan
Tutor: WangYuanYing
School: Chinese Academy of Agricultural Sciences
Course: Crop Genetics and Breeding
Keywords: Tobacco Cucumber mosaic virus Genetic analysis Molecular markers
CLC: S572
Type: Master's thesis
Year: 2009
Downloads: 108
Quote: 0
Read: Download Dissertation

Abstract


Tobacco is one of the important economic crops in China.Tobacco cucumber mosaic virus （CMV） is one of the most ubiquitous plant virus in the world and it has various stains,which caused substantive loss to tobacco production.Besides the yield loss,the more serious thing is its effects on color and aroma of tobacco leaves,which can reduce its commercial value significantly.The main content include three aspects as follows:（1） The disease resistance was evaluated by artificial inoculation on seedlings in greenhouse and the inheritance of resistance was analyzed; （2） The orthogonal design was used to establish the reaction system of tobacco SSR molecular markers;（3） Bulked segregant analysis was used to screen the SSR molecular markers of tobacco CMV resistance gene for seeking molecular markers linked to the CMV resistance gene.The main results are as follows:（1） Taiyan-8 was highly resistant to CMV and tiebazi has moderate resistance,Both of the anti-sence of F1 were susceptible to CMV,which means the resistant character of tested materials assumed a recessive heredity.The anti-sence segregation ratio of F2 generation were 3:1 and BC1 generation were 1:1.x2 test showed that there are no significant differences between measured value and theoretical value both in F2 and BC1 generation.Therefore we came to the conclusion that the resistant character of tiebazi and taiyan-8 was probably controlled by single recessive genes.（2） The best reaction system of the tobacco SSR-PCR was established.Taking advantage of this system on the PCR amplification process of annealing temperature and cycle times for the screening, we obtained the best response procedures.（3） DNAs of F2 population were extracted to develop into a resistant pool and a sensitive pool based on the BSA（bulk segregant analysis）.182 pairs SSR primers were used for molecular marking analysis.The results showed that the genetic distance between SM1 and resistant genes from“tiebazi”is 8.64 cM,and the genetic distance between SM2 and resistant genes from“taiyan-8”is 3.92 cM.

Related Dissertations

  1. Application of Numerical Designing Cigarette Blending Formula on Computational Intelligence,TS44
  2. Preparation of Activated Carbon from Tobacco Stems and Application for Low Concentration Phosphine Adsorption,X712
  3. Tobacco Diseases Auto-Recognition Research Based on Image Processing Technology,S435.72
  4. Effect of Water and Nitrogen Coupling Using on Soil Physical and Chemical Properties in Tobacco Areas and the Yield and Quality of Fule-cured Tobacco in Yibin,S572
  5. The Distribution Characteristics of Osmiophilic Granules in Tobacco Leaves at Different Altitudes and the Relationship with the Quality of Flue-cured Tobacco Leaves of Huili,S572
  6. Color Parameters Changes of Flue-cured Tobacco during Maturing and Curing and the Influence of Air-curing Before Flue-curing on Its Quality,S572
  7. The Effect of Frequencycontron on Flow Fields Environment in Bulk Curing Barn and Quality of Flue Cured Tobacco,S572
  8. Analysis on Characteristics and Discriminant of Aroma Types of Flue-Cured Tobacco,S572
  9. Analysis of Attributes of Quality and Soil Factors on Style of Fen-flavor Flue-cured Tobacco in Qujing District,S572
  10. Influences of Density and Potassium Interaction on Physio-ecological Indicators and Quality of Flue-cured Tobacco in Yanbian,S572
  11. The Study on Rules of Carbon and Nitrogen Metabolisms and Its Relationship with Flavor Quality of Strong Aroma Style Flue-cured Tobacco in Henan,S572
  12. Genetic Analysis and Mapping of the Mutant Locus of a Dwarf and Compact Spike Mutant NAUH164 from Wheat Variety Sumai 3,S512.1
  13. Fine Mapping of Two Leaf Mutant Genes al and yl in Maize,S513
  14. Characterization and QTL Mapping of a High-tillering and Dwarf Mutant of Wheat Variety Wangshuibai,S512.1
  15. Genetic Analysis and QTL Mapping of Dwarf and Compact Spike Mutant of Sumai 3,S512.1
  16. Study on Synthesis of Xyloses Esters and Their Application in Cigarette,TQ655
  17. Study on the Evaluation Method of Flue-cured Tobacco Sensory Characters,S572
  18. Flexible resource scheduling algorithm for dynamic combinatorial production and realization,F426.8
  19. Analysis of Resource and Genetic Diversity of Wild Walnut in Xinjiang,S664.1
  20. NGS Yunnan Tobacco Logistics Integration,F426.8
  21. Identification of Aroma Active Compounds in Essential Oils and Tobacco Leaves Using Gase Chromatography-Mass Spectremetry/Olfactometry (GC-MS/O),TS411

CLC: > Agricultural Sciences > Crop > Economic crops > Tobacco ( tobacco )
© 2012 www.DissertationTopic.Net  Mobile